Knockdown of PKC[epsilon] Expression Inhibits Growth, Induces Apoptosis and Decreases Invasiveness of Human Glioma Cells Partially Through Stat3

Glioma is the most common primary central nervous system tumor. Despite considerable research effort, little progress has been made in the therapeutic treatment of this disease. Protein kinase C[straight epsilon] (PKC[straight epsilon]), an important intracellular signaling molecule, modulates diver...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2015-01, Vol.55 (1), p.21
Main Authors: Xu, Yaming, Li, Zhe, Zhang, Can, Zhang, Shiyun, Ji, Yonghua, Chen, Fuxue
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Brain cancer
Cancer therapies
Cell cycle
Cell growth
Kinases
Localization
Medical prognosis
Nervous system
Phosphorylation
Proteins
Tumors
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Summary:Glioma is the most common primary central nervous system tumor. Despite considerable research effort, little progress has been made in the therapeutic treatment of this disease. Protein kinase C[straight epsilon] (PKC[straight epsilon]), an important intracellular signaling molecule, modulates diverse cellular functions, including cell proliferation, apoptosis, invasion and differentiation. The aim of the study is to investigate whether knockdown of PKC[straight epsilon] expression by RNA interference (RNAi) could affect the growth, apoptosis and invasion of human glioma cells, and the involvement of the signal transducer and activator of transcription 3 (Stat3) signaling pathway in these effects. Our data showed that knockdown of PKC[straight epsilon] expression inhibited proliferation, induced apoptosis and decreased invasiveness of human glioma cell lines U251 and U87, as well as suppressed the growth of U87 cell-derived tumors in nude mice. Moreover, PKC[straight epsilon] physically interacts with Stat3, and knockdown of PKC[straight epsilon] expression attenuated Stat3Ser727 phosphorylation and B-cell lymphoma-extra large (Bcl-xL) expression in the two human glioma cell lines. These results support an important role for PKC[straight epsilon] in glioma cell growth, apoptosis and invasion, and PKC[straight epsilon] exerting its above effects at least in part through Stat3. Thus, PKC[straight epsilon] has the potential to be an attractive therapeutic target for glioma therapy.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-014-0341-4