Antigen-specific T-cell responses in patients with non–IgE-mediated gastrointestinal food allergy are predominantly skewed to TH2

The limulus amebocyte lysate assay detected high concentrations of LPS in commercially available milk protein preparations, as previously reported (see Table E1 in this article's Online Repository at www.jacionline.org).3 In addition, significant lymphoproliferative responses were found in the...

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Published in:Journal of allergy and clinical immunology 2013-02, Vol.131 (2), p.590-592.e6
Main Authors: Morita, Hideaki, Nomura, Ichiro, Orihara, Kanami, Yoshida, Koichi, Akasawa, Akira, Tachimoto, Hiroshi, Ohtsuka, Yoshikazu, Namai, Yoshiyuki, Futamura, Masaki, Shoda, Tetsuo, Matsuda, Akio, Kamemura, Norio, Kido, Hiroshi, Takahashi, Takao, Ohya, Yukihiro, Saito, Hirohisa, Matsumoto, Kenji
Format: Article
Language:English
Subjects:
Allergies
Cytokines
Food allergies
Milk
Studies
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Summary:The limulus amebocyte lysate assay detected high concentrations of LPS in commercially available milk protein preparations, as previously reported (see Table E1 in this article's Online Repository at www.jacionline.org).3 In addition, significant lymphoproliferative responses were found in the presence of as little as 10 pg/mL LPS (see Fig E1, A, in this article's Online Repository at www.jacionline.org), and PBMCs from younger children showed more pronounced lymphoproliferation in response to LPS (see Fig E1, B). [...]we attempted to remove contaminating LPS from milk protein preparations by passing them through a prepacked endotoxin affinity column. Â Proliferation (SI)[low *] 20 1.290 (0.830-1.738) 9 3.077 (2.484-3.492) 65 2.894 (2.004-7.147)
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2012.09.005