EncapsulatedBifidobacteriareduced bacterial translocation in rats following hemorrhagic shock and resuscitation

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2007-10, Vol.23 (10), p.754
• Main Authors: Ruan, Xiangcai, Shi, Hanping, Xia, Gengfeng, Xiao, Ying, Dong, Jiaxi, Ming, Feiping, Wang, Shenming
• Format: Article
• Language: English
• Subjects: Bacteria; Blood pressure; Catheters; Disease; Endotoxins; Experiments; Laboratory animals; Lymph nodes; Lymphatic system; Microbiological analysis; Multiple organ dysfunction syndrome; Probiotics; Rodents; Sepsis; Translocation
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2007.07.002

Objective The aim of the present study was to determine the effects of peroral encapsulatedBifidobacteriaon intestinal microflora, bacterial translocation (BT), plasma endotoxin, and ileal villi injury in a rat model of hemorrhagic shock. Methods Sprague-Dawley rats were fed daily with three different diet supplements: phosphate buffered saline,Bifidobacteria(109colon-forming units/day), or microencapsulatedBifidobacteria(109colony-forming units/day). After 7 d of treatment, rats were anesthetized for hemorrhagic or sham shock. Then a laparotomy was performed to determine microbiological analysis of cecal content, BT to mesenteric lymph nodes, plasma endotoxin, and terminal ileal villous damage. Results In the hemorrhagic-shock model, rats pretreated withBifidobacteriashowed decreases in total aerobes in cecum, magnitude of total aerobes to BT, levels of plasma endotoxin, and percentage of ileal villous damage when compared with rats treated with phosphate buffered saline. EncapsulatedBifidobacteriainduced greater decreases than intactBifidobacteriain this model, except for no difference in percentage of ileal villous damage between the two groups. In addition, the incidence of BT was decreased in hemorrhagic rats pretreated withBifidobacteriacompared with control. However, the magnitude of total anaerobes andBifidobacteriaBT were similar among hemorrhagic-shocked rats receiving three different supplements. Conclusion Bifidobacteriacan be useful in preventing BT in hemorrhagic-shocked rats, and encapsulatedBifidobacteriacan augment this effect further. Peroral administration ofBifidobacteriamay be a favorable strategy to prevent sepsis and multiple organ dysfunction syndrome in hemorrhagic shock.