Multiple resistance across glufosinate, glyphosate, paraquat and ACCase‐inhibiting herbicides in an Eleusine indica population

• Published in: Weed research 2015-02, Vol.55 (1), p.82-89
• Main Authors: Jalaludin, A, Yu, Q, Powles, S B, Darmency, Henri
• Format: Article
• Language: English
• Subjects: at-risk population; dose response; Eleusine indica; fluazifop-P; fluazifop-P-butyl; genes; glufosinate; glyphosate; herbicide resistance; Herbicides; Indian goosegrass; mutation; non-selective herbicides; paraquat; Population; weeds
• ISSN: 0043-1737; 1365-3180
• DOI: 10.1111/wre.12118

An Eleusine indica population was previously reported as the first global case of field‐evolved glufosinate resistance. This study re‐examines glufosinate resistance and investigates multiple resistance to other herbicides in the population. Dose–response experiments with glufosinate showed that the resistant population is 5‐fold and 14‐fold resistant relative to the susceptible population, based on GR₅₀and LD₅₀R/S ratio respectively. The selected glufosinate‐resistant subpopulation also displayed a high‐level resistance to glyphosate, with the respective GR₅₀and LD₅₀R/S ratios being 12‐ and 144‐fold. In addition, the subpopulation also displayed a level of resistance to paraquat and ACCase‐inhibiting herbicides fluazifop‐P‐butyl, haloxyfop‐P‐methyl and butroxydim. ACCase gene sequencing revealed that the Trp‐2027‐Cys mutation is likely responsible for resistance to the ACCase inhibitors examined. Here, we confirm glufosinate resistance and importantly, we find very high‐level glyphosate resistance, as well as resistance to paraquat and ACCase‐inhibiting herbicides. This is the first confirmed report of a weed species that evolved multiple resistance across all the three non‐selective global herbicides, glufosinate, glyphosate and paraquat.