Effects of La(III) and Ca(II) on Isolated Carassius auratus Liver Mitochondria: Heat Production and Mitochondrial Permeability Transition

• Published in: Biological trace element research 2015-02, Vol.163 (1-2), p.217-223
• Main Authors: Wu, Man, Gao, Jia-Ling, Sun, Mei-Xiang, Zhang, Ye-Zhong, Liu, Yi, Dai, Jie
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biotechnology; Calcium; Calcium - pharmacology; Goldfish - metabolism; Heat; Lanthanum; Lanthanum - pharmacology; Life Sciences; Liver; Membrane Potential, Mitochondrial - drug effects; Mitochondria; Mitochondria, Liver - metabolism; Mitochondrial Membranes - metabolism; Nutrition; Oncology; Permeability
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-014-0178-2

The effects of lanthanum and calcium on heat production of mitochondria isolated from Carassius auratus liver were investigated by microcalorimetry, and their effects on mitochondrial swelling and membrane potential (Δ ψ ) were determined by spectroscopic methods. La 3+ showed only inhibitory action on mitochondrial energy turnover with inhibition concentration of 50 % (IC 50 ) being 71.2 μmol L −1 . Similarly, Ca 2+ restrained the heat production of mitochondria, and the IC 50 of Ca 2+ was much higher than that of La 3+ . In the spectroscopic experiments, La 3+ and Ca 2+ induced fish liver mitochondrial swelling and decreased membrane potential (Δ ψ ), and the induction ability of La 3+ was stronger than that of Ca 2+ . It is concluded that the effects of La 3+ and Ca 2+ on fish liver mitochondria differ, and La represents toxic action rather than Ca analogy.