The role of endothelial nitric oxide synthase gene G894T and intron 4 VNTR polymorphisms in hemodialysis patients with vascular access thrombosis

• Published in: Anadolu kardiyoloji dergisi : AKD 2014-05, Vol.14 (3), p.239-243
• Main Authors: Şener, Elif Funda, Emiroğulları, Ömer Naci, Serhatlıoğlu, Faruk, Özkul, Yusuf
• Format: Article
• Language: English
• Subjects: Case-Control Studies; Cholesterol - blood; European Continental Ancestry Group; Female; Genetic Predisposition to Disease; Humans; Introns - genetics; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - genetics; Kidney Failure, Chronic - therapy; Male; Middle Aged; Nitric Oxide Synthase Type III - genetics; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Renal Dialysis - adverse effects; Thrombosis - complications; Turkey; Vascular Access Devices
• ISSN: 1302-8723; 2149-2263; 1308-0032; 2149-2271
• DOI: 10.5152/akd.2013.4779

Endothelial nitric oxide synthase (eNOS) gene is a candidate gene in cardiovascular and renal diseases. Several polymorphic variations have been identified in eNOS gene. We investigated a potential role of arteriovenous fistula (AVF) thrombosis and intron 4 and G894T polymorphisms in chronic renal failure. We performed a case-control observational study involving 79 with/without AVF thrombosis in chronic renal failure patients. All subjects were genotyped by the polymerase chain reaction (PCR) and PCR-Restriction Fragment Length Polymorphism. Genotype distribution and allele frequencies were compared between groups using the chi-square test. Genotype frequencies in patients with thrombosis were not significantly different from those of patients without thrombosis for eNOS G894T polymorphism (p=0.1). eNOS gene intron 4 a allele distributions seems to be associated with thrombosis in the groups. This study revealed that there was an association between eNOS intron 4 polymorphism and thrombosis in chronic renal failure patients. This data will be helpful in planning further eNOS association studies in vascular access thrombosis.