xMAP-based analysis of three most prevalent staphylococcal toxins in Staphylococcus aureus cultures

Detection of staphylococcal toxins presents a great interest for medical diagnostics. Screening of clinical samples for the presence of several types of staphylococcal toxins using traditional methods—biological tests on animals or cell cultures as well as ELISA—is laborious. Multiplex detection met...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2014-10, Vol.406 (25), p.6447-6452
Main Authors: Simonova, Maria A, Petrova, Elena. E, Dmitrenko, Olga A, Komaleva, Ravilya L, Shoshina, Natalia S, Samokhvalova, Larisa V, Valyakina, Tatiana I, Grishin, Eugene V
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
bacteria
bacterial toxins
Biochemistry
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
culture media
Culture Media - analysis
detection limit
diagnostic techniques
enterotoxins
Epidemiology
Exotoxins - analysis
Exotoxins - metabolism
Food Science
foods
Immunoassay
Immunoassay - methods
Ionization
Laboratory Medicine
Mass spectrometry
monitoring
Monitoring/Environmental Analysis
Monoclonal antibodies
Nanotechnology
natural toxicants
Nosocomial infections
Research centers
screening
septic shock
Staphylococcus aureus
Staphylococcus aureus - chemistry
Staphylococcus aureus - growth & development
Staphylococcus aureus - metabolism
Staphylococcus infections
Toxins
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Summary:Detection of staphylococcal toxins presents a great interest for medical diagnostics. Screening of clinical samples for the presence of several types of staphylococcal toxins using traditional methods—biological tests on animals or cell cultures as well as ELISA—is laborious. Multiplex detection methods would simplify testing. We have designed an xMAP-based assay to detect three staphylococcal toxins—enterotoxins A and B (SEA and SEB) and toxic shock syndrome toxin (TSST)—in cultural supernatants obtained from different strains of Staphylococcus aureus. The limits of detection of SEA, SEB, and TSST multiplex detection in S. aureus growth medium were 10, 1,000, and 5 pg/mL, respectively. Fifty-nine samples of S. aureus cultural supernatants were tested with the xMAP assay. The developed assay has proved highly effective detection of the natural toxins in the samples obtained due to bacterial cells cultivation. In prospect, the developed test system can be used in clinical diagnostics and in monitoring of foodstuffs and environmental objects.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-8048-5