Rapid Synthesis of Cyclic RGD Conjugated Gold Nanoclusters for Targeting and Fluorescence Imaging of Melanoma A375 Cells

A simple, rapid, and inexpensive method for the synthesis of cyclic arginine-glycine-aspartic acid (RGD) peptide conjugated gold nanoclusters (RGD-GNCs) was reported. The nanoclusters were synthesized with chloraurate as precursor and cyclic RGD peptides as both reducing and protecting agent directl...

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Bibliographic Details
Published in:Bioconjugate chemistry 2015-02, Vol.26 (2), p.243-249
Main Authors: Yin, Hua-Qin, Bi, Feng-Li, Gan, Feng
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cells
Fluorescence
Gold - chemistry
Humans
Integrin alphaVbeta3 - analysis
MCF-7 Cells
Melanoma - diagnosis
Molecules
Nanostructures - chemistry
Nanostructures - ultrastructure
Optical Imaging - methods
Peptides
Peptides, Cyclic - chemical synthesis
Peptides, Cyclic - chemistry
Photoelectron Spectroscopy
Spectroscopy, Fourier Transform Infrared
Toxicity
Transmission electron microscopy
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Summary:A simple, rapid, and inexpensive method for the synthesis of cyclic arginine-glycine-aspartic acid (RGD) peptide conjugated gold nanoclusters (RGD-GNCs) was reported. The nanoclusters were synthesized with chloraurate as precursor and cyclic RGD peptides as both reducing and protecting agent directly under alkali condition, and the whole synthetic process only took 15 min at room temperature. The properties of the nanoclusters were characterized by means of ultraviolet–visible spectra, Fourier transform infrared spectroscopy (FTIR), fluorescence, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The prepared gold nanoclusters were successfully used as a contrast agent in fluorescence imaging of the melanoma A375 cells, which overexpress the integrin αvβ3. The results demonstrated that our nanoclusters possess good biocompatibility, stability, and low toxicity. Moreover, the method is expected to be applicable to the synthesis of nanoclusters conjugated with other biomolecules.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc500505c