Bursaphelenchus fungivorus from Pinus pinaster bark in Portugal

Bursaphelenchus fungivorus is reported for the first time in Portugal, identified as associated with Pinus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism...

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Bibliographic Details
Published in:Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie 2014-04, Vol.44 (2), p.131-136
Main Authors: Fonseca, L, Cardoso, J. M. S, Moron‐Lopez, J, Abrantes, I, Sturrock, R. N
Format: Article
Language:English
Subjects:
bark
Bursaphelenchus
genetic variation
internal transcribed spacers
Pinus pinaster
restriction fragment length polymorphism
ribosomal DNA
sequence alignment
taxonomy
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Summary:Bursaphelenchus fungivorus is reported for the first time in Portugal, identified as associated with Pinus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA. Intraisolate genetic variability was detected among ITS sequences of the Portuguese B. fungivorus isolate. Phylogenetic analysis, obtained from multiple sequence alignment between ITS sequences of Bursaphelenchus species, revealed that the Portuguese B. fungivorus isolate clusters with other B. fungivorus isolates, forming a separate group close to B. seani highlighting a molecular proximity of these two species.
ISSN:1437-4781
1439-0329
DOI:10.1111/efp.12077