Determination of Sex Origin of Meat and Meat Products on the DNA Basis: A Review

Sex determination of domestic animal's meat is of potential value in meat authentication and quality control studies. Methods aiming at determining the sex origin of meat may be based either on the analysis of hormone or on the analysis of nucleic acids. At the present time, sex determination o...

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Bibliographic Details
Published in:Critical reviews in food science and nutrition 2015-01, Vol.55 (10), p.1303-1314
Main Authors: Gokulakrishnan, Palanisamy, Kumar, Rajiv Ranjan, Sharma, Brahm Deo, Mendiratta, Sanjod Kumar, Malav, Omprakash, Sharma, Deepak
Format: Article
Language:English
Subjects:
Animals
authentication
Chromatography
Deoxyribonucleic acid
DNA
DNA - analysis
domestic animals
Electrophoresis
enzyme-linked immunosorbent assay
Female
Food science
Gas chromatography
gas-chromatography-mass spectrometry
gel electrophoresis
Hormones
liquid chromatography
Male
Mass spectrometry
Meat
Meat - analysis
Meat products
Meat Products - analysis
nutrition science
Origins
PCR
Polymerase Chain Reaction
quality control
Sex
Sex determination
Sex Determination Analysis
sexing
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Summary:Sex determination of domestic animal's meat is of potential value in meat authentication and quality control studies. Methods aiming at determining the sex origin of meat may be based either on the analysis of hormone or on the analysis of nucleic acids. At the present time, sex determination of meat and meat products based on hormone analysis employ gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS), and enzyme-linked immunosorbent assay (ELISA). Most of the hormone-based methods proved to be highly specific and sensitive but were not performed on a regular basis for meat sexing due to the technical limitations or the expensive equipments required. On the other hand, the most common methodology to determine the sex of meat is unquestionably traditional polymerase chain reaction (PCR) that involves gel electrophoresis of DNA amplicons. This review is intended to provide an overview of the DNA-based methods for sex determination of meat and meat products.
ISSN:1549-7852
1040-8398
1549-7852
DOI:10.1080/10408398.2012.690095