Comparison of four molecular methods to type Salmonella Enteritidis strains

This study compared the pulsed‐field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus‐PCR (ERIC‐PCR), multilocus variable‐number of tanden‐repeat analysis (MLVA), and multilocus sequence typing (MLST) methods for typing 188 Salmonella Enteritidis strains from different sou...

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Bibliographic Details
Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2015-05, Vol.123 (5), p.422-426
Main Authors: Campioni, Fábio, Pitondo-Silva, André, Bergamini, Alzira M.M., Falcão, Juliana P.
Format: Article
Language:English
Subjects:
Animals
Brazil - epidemiology
Chickens - microbiology
Eggs
Electrophoresis, Gel, Pulsed-Field - methods
Epidemics
ERIC-PCR
Food Microbiology
Humans
Methods
Minisatellite Repeats
MLST
MLVA
molecular epidemiology
Molecular Epidemiology - methods
Molecular Typing - methods
Multilocus Sequence Typing - methods
PFGE
Polymerase Chain Reaction - methods
Poultry
Salmonella
Salmonella Enteritidis
Salmonella enteritidis - classification
Salmonella enteritidis - genetics
Salmonella enteritidis - isolation & purification
Salmonella Food Poisoning - epidemiology
Salmonella Food Poisoning - microbiology
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Summary:This study compared the pulsed‐field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus‐PCR (ERIC‐PCR), multilocus variable‐number of tanden‐repeat analysis (MLVA), and multilocus sequence typing (MLST) methods for typing 188 Salmonella Enteritidis strains from different sources isolated over a 24‐year period in Brazil. PFGE and ERIC‐PCR were more efficient than MLVA for subtyping the strains. However, MLVA provided additional epidemiological information for those strains. In addition, MLST showed the Brazilian strains as belonging to the main clonal complex of S. Enteritidis, CC11, and provided the first report of two new STs in the S. enterica database but could not properly subtype the strains. Our results showed that the use of PFGE or ERIC‐PCR together with MLVA is suitable to efficiently subtype S. Enteritidis strains and provide important epidemiological information.
ISSN:0903-4641
1600-0463
DOI:10.1111/apm.12367