Estandarizacion de un protocolo de regeneracion in vitro para pitahaya amarilla (Selenicereus megalanthus (K. Schum. ex Vaupel) Moran)

A protocol for in vitro regeneration in yellow pitahaya by using indirect organogenesis pathway was standardized from axillary meristem grown on MS medium. The medium was supplemented with three concentrations of 2,4-D (2.26, 3.26 and 4.26 pM) or 2,4-D + 6- BAP to 2.21 mM, or with three concentratio...

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Bibliographic Details
Published in:Acta agronomica (Palmira) 2014-01, Vol.63 (1), p.31
Main Authors: Diego Geraldo Caetano Nunes, Escobar, Roosevelt, Creuci Maria Caetano, Vaca Vaca, Juan Carlos
Format: Article
Language:eng ; spa
Subjects:
Agronomy
Fruits
Plant growth
Plant propagation
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Summary:A protocol for in vitro regeneration in yellow pitahaya by using indirect organogenesis pathway was standardized from axillary meristem grown on MS medium. The medium was supplemented with three concentrations of 2,4-D (2.26, 3.26 and 4.26 pM) or 2,4-D + 6- BAP to 2.21 mM, or with three concentrations of TDZ (200, 300 and 400 pM) or TDZ + 6- BAP, a total of 12 treatments. Growing conditions evaluated included the use of light (photoperiod of 16/8) and darkness (0/24). The 2.4-D induced calli but regeneration efciency for the species was nil. In turn, the TDZ or TDZ + BAP were more efcient in inducing compact calli, green-purple, with capacity of regeneration following indirect organogenesis. With 300pM of TDZ more efciency of response was observed, because the number of buds formed by regeneration point was the highest. Histological analysis conrmed the route of regeneration in S. megalanthus, showing primary structures characteristic of the formation of shoots from calli.
ISSN:0120-2812
2323-0118