Análisis genómico del resistoma de la cepa de Acinetobacter baumannii ABIBUN 107m multi-resistente y persistente en hospitales colombianos

Acinetobacter baumannii is a bacterium causing health care associated infections such as pneumonia, septicemia, meningitis and urinary infections amongst others. It has great capacity to quickly develop and gather a big variety of drug resistance mechanisms. In this research, the genome of strain A....

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Bibliographic Details
Published in:Revista Colombiana de biotecnologia 2014-12, Vol.16 (2), p.104-113
Main Authors: Reguero, Maria Teresa, Mantilla, José Ramón, Valenzuela de Silva, Emilia María, Falquet, Laurent, González, Elsa Beatriz, Flores, Vanessa, Uribe, Laura Patricia, Barreto Hernández, Emiliano
Format: Article
Language:eng ; spa
Subjects:
Bacteria
Genomes
Health care
Infections
Microorganisms
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Summary:Acinetobacter baumannii is a bacterium causing health care associated infections such as pneumonia, septicemia, meningitis and urinary infections amongst others. It has great capacity to quickly develop and gather a big variety of drug resistance mechanisms. In this research, the genome of strain A. baumannii ABIBUN 107m was analyzed wich forms part of a persistent clon in Colombian hospitals and it's also resistant to carbapenems (imipenem and meropenem), which are the election antibiotics for treatment of infections caused by this microorganism. The genome was sequenced using high performance technology, assembled and annotated. As a result, we obtained a 3954000 bp genome, with 56 contigs; 4256 genes with average size of 912 bp; 3796 CDS; 2884 were assigned to COG; 57 tRNA and GC percentage of 38,74%. The A. baumannii strain ABIBUN 107m, is resistant to the following antibiotic groups: b-lactams, aminoglycosides, quinolones, tetracycline, sulfonamide and colistin. Genes associated with this resistance profile were found in A. baumannii ABIBUN 107m genome serino b-lactamases (blaADC-38, blaOXA-64, blaOXA-23, blaampC-like, blaamp(H)-like, metallo b-lactamase_B; High Molecular Mass penicillin binding proteins, ISAba1 type insertion sequences, mutations of DNA gyrase and topoisomerase IV subunit A (gyrA and parC); aminoglycoside modifying enzymes (aphA-like, aadA-like); choramphenicol acyltransferase (cat) and dehydropteroate synthase (sul-1). Genes belonging to five different efflux systems were identified (RND, MATE, MSF, ATP, SMR).
ISSN:0123-3475
1909-8758
DOI:10.15446/rev.colomb.biote.v16n2.47247