Role of the cholinergic anti-inflammatory pathway in endotoxemia-induced multi-organ damage in the rat

Objective: To investigate the role of the cholinergic anti-inflammatory pathway and interaction of inducible nitric oxide (iNOS) and cycloxygenase (COX)-2 in organ dysfunction in rat sepsis. Methods: Lipopolysaccharide (LPS) (10 mg/kg; intraperitoneally) was given to Sprague-Dawley rats to induce se...

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Published in:Clinical and experimental health sciences (Online) 2015, Vol.5 (1), p.1
Main Authors: Kolgazi, Meltem, Ozgur, Fatih, Beyazoglu, Omer, Kocagoz, Ali, Celik, Mehmethan, Elmas, Merve, Yuksel, Meral, Ercan, Feriha, Alican, Inci
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Language:eng ; tur
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Summary:Objective: To investigate the role of the cholinergic anti-inflammatory pathway and interaction of inducible nitric oxide (iNOS) and cycloxygenase (COX)-2 in organ dysfunction in rat sepsis. Methods: Lipopolysaccharide (LPS) (10 mg/kg; intraperitoneally) was given to Sprague-Dawley rats to induce sepsis. Groups were treated with nicotine alone or with nicotinic receptor antagonist mecamylamine (3 mg/kg; subcutaneously), selective iNOS inhibitor aminoguanidine (8 mg/kg; intraperitoneally) or selective COX-2 inhibitor nimesulide (8 mg/kg; intraperitoneally). Six hours after LPS, liver, kidney and lung samples were obtained for malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO), chemiluminescence assays and microscopic evaluation and blood was obtained for alanine transaminase (ALT), aspartate aminotransferase (AST) and blood urea nitrogen (BUN) assays. Results: Sepsis increased ALT (p
ISSN:2146-3654
2146-3654
2459-1459
DOI:10.5455/musbed.20150306053623