Potential anti-angiogenic, antiproliferative, antioxidant, and anticoagulant activity of anionic polysaccharides, fucans, extracted from brown algae Lobophora variegata

This study analyzed the action of sulfated polysaccharides, fucans, from the brown alga Lobophora variegata on angiogenesis (CAM assays), antioxidant activity, and homeostasis in vitro. Groups of fucans, obtained after acetone fractionation in several (0.3–1.5 v) concentrations, were obtained. This...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied phycology 2015-06, Vol.27 (3), p.1315-1325
Main Authors: Castro, Luiza Sheyla Evenni Porfírio Will, de Sousa Pinheiro, Thuane, Castro, Allisson Jhonatan Gomes, da Silva Nascimento Santos, Marilia, Soriano, Eliane Marinho, Leite, Edda Lisboa
Format: Article
Language:English
Subjects:
Acetone
agarose
Algae
angiogenesis
anticoagulant activity
Anticoagulants
antioxidant activity
Antioxidants
Biomedical and Life Sciences
carcinoma
chemical analysis
Ecology
electrophoresis
Fractionation
free radicals
Freshwater & Marine Ecology
homeostasis
Life Sciences
liver
neoplasm cells
Phaeophycophyta
Plant Physiology
Plant Sciences
Saccharides
thromboplastin
viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783
cites cdi_FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783
container_end_page 1325
container_issue 3
container_start_page 1315
container_title Journal of applied phycology
container_volume 27
creator Castro, Luiza Sheyla Evenni Porfírio Will
de Sousa Pinheiro, Thuane
Castro, Allisson Jhonatan Gomes
da Silva Nascimento Santos, Marilia
Soriano, Eliane Marinho
Leite, Edda Lisboa
description This study analyzed the action of sulfated polysaccharides, fucans, from the brown alga Lobophora variegata on angiogenesis (CAM assays), antioxidant activity, and homeostasis in vitro. Groups of fucans, obtained after acetone fractionation in several (0.3–1.5 v) concentrations, were obtained. This sulfated heterofucan was eluted with 0.8 v of acetone named fraction rich in fucans (FRF). The presence of polysaccharides had its homogeneity confirmed by electrophoresis in agarose with buffer 1,3-diaminopropane and sulfate groups by chemical analyses. We observed that FRF showed antioxidant activity with strong inhibition of hydroxyl and strong inhibition of peroxide. FRF at 25 μg mL⁻¹decreased the cellular viability (54.2 ± 0.2 %) using the MTT assay in tumor cell line HepG2 (hepatocellular liver carcinoma cell line). This polysaccharide exhibited anti-angiogenic effect and anticoagulant activity on intrinsic pathway (activated partial thromboplastin time (aPTT). The results showed that FRF from L. variegata has significant pharmacological activity in models of anticoagulation, angiogenesis, and inhibition of free radicals.
doi_str_mv 10.1007/s10811-014-0424-1
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1679017997</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3676570571</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783</originalsourceid><addsrcrecordid>eNp9kUFv1DAQhSMEEkvbH8AJS1zrMuPEcXxEFQWklUBqe7bGjpO6SuPFzrbdf8TPxNtw4MTp2c_vG2v0quo9wgUCqE8ZoUPkgA2HRjQcX1UblKrmElX7utqAFsg7rfBt9S7newDQHXab6vfPuPh5CTQxKsJpHkMc_Rzc-YuxS3EKg0-0hEe_WvE59EWPl_7FcJHG_VROjFyJheXA4lBeQixj2C5Oh0zO3VEKvc_nbNg7mov65yUVwPdsSPGB2RSfZkbTSJ5to427u5iIPRbKj7TQafVmoCn7s796Ut1efbm5_Ma3P75-v_y85a5BWHjb9dJabR3U2lsntGzbulUAUjnprBwQtWg0idYqWQ-is8I2XV8MD96rrj6pPq5zy-a_9j4v5j7u01y-NNgqDai0ViWFa8qlmHPyg9ml8EDpYBDMsRCzFmJKIeZYiMHCiJXJJTuPPv0z-T_QhxUaKBoaU8jm9loASgBRVhS6_gNElJr_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1679017997</pqid></control><display><type>article</type><title>Potential anti-angiogenic, antiproliferative, antioxidant, and anticoagulant activity of anionic polysaccharides, fucans, extracted from brown algae Lobophora variegata</title><source>Springer Link</source><creator>Castro, Luiza Sheyla Evenni Porfírio Will ; de Sousa Pinheiro, Thuane ; Castro, Allisson Jhonatan Gomes ; da Silva Nascimento Santos, Marilia ; Soriano, Eliane Marinho ; Leite, Edda Lisboa</creator><creatorcontrib>Castro, Luiza Sheyla Evenni Porfírio Will ; de Sousa Pinheiro, Thuane ; Castro, Allisson Jhonatan Gomes ; da Silva Nascimento Santos, Marilia ; Soriano, Eliane Marinho ; Leite, Edda Lisboa</creatorcontrib><description>This study analyzed the action of sulfated polysaccharides, fucans, from the brown alga Lobophora variegata on angiogenesis (CAM assays), antioxidant activity, and homeostasis in vitro. Groups of fucans, obtained after acetone fractionation in several (0.3–1.5 v) concentrations, were obtained. This sulfated heterofucan was eluted with 0.8 v of acetone named fraction rich in fucans (FRF). The presence of polysaccharides had its homogeneity confirmed by electrophoresis in agarose with buffer 1,3-diaminopropane and sulfate groups by chemical analyses. We observed that FRF showed antioxidant activity with strong inhibition of hydroxyl and strong inhibition of peroxide. FRF at 25 μg mL⁻¹decreased the cellular viability (54.2 ± 0.2 %) using the MTT assay in tumor cell line HepG2 (hepatocellular liver carcinoma cell line). This polysaccharide exhibited anti-angiogenic effect and anticoagulant activity on intrinsic pathway (activated partial thromboplastin time (aPTT). The results showed that FRF from L. variegata has significant pharmacological activity in models of anticoagulation, angiogenesis, and inhibition of free radicals.</description><identifier>ISSN: 0921-8971</identifier><identifier>EISSN: 1573-5176</identifier><identifier>DOI: 10.1007/s10811-014-0424-1</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Acetone ; agarose ; Algae ; angiogenesis ; anticoagulant activity ; Anticoagulants ; antioxidant activity ; Antioxidants ; Biomedical and Life Sciences ; carcinoma ; chemical analysis ; Ecology ; electrophoresis ; Fractionation ; free radicals ; Freshwater &amp; Marine Ecology ; homeostasis ; Life Sciences ; liver ; neoplasm cells ; Phaeophycophyta ; Plant Physiology ; Plant Sciences ; Saccharides ; thromboplastin ; viability</subject><ispartof>Journal of applied phycology, 2015-06, Vol.27 (3), p.1315-1325</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783</citedby><cites>FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Castro, Luiza Sheyla Evenni Porfírio Will</creatorcontrib><creatorcontrib>de Sousa Pinheiro, Thuane</creatorcontrib><creatorcontrib>Castro, Allisson Jhonatan Gomes</creatorcontrib><creatorcontrib>da Silva Nascimento Santos, Marilia</creatorcontrib><creatorcontrib>Soriano, Eliane Marinho</creatorcontrib><creatorcontrib>Leite, Edda Lisboa</creatorcontrib><title>Potential anti-angiogenic, antiproliferative, antioxidant, and anticoagulant activity of anionic polysaccharides, fucans, extracted from brown algae Lobophora variegata</title><title>Journal of applied phycology</title><addtitle>J Appl Phycol</addtitle><description>This study analyzed the action of sulfated polysaccharides, fucans, from the brown alga Lobophora variegata on angiogenesis (CAM assays), antioxidant activity, and homeostasis in vitro. Groups of fucans, obtained after acetone fractionation in several (0.3–1.5 v) concentrations, were obtained. This sulfated heterofucan was eluted with 0.8 v of acetone named fraction rich in fucans (FRF). The presence of polysaccharides had its homogeneity confirmed by electrophoresis in agarose with buffer 1,3-diaminopropane and sulfate groups by chemical analyses. We observed that FRF showed antioxidant activity with strong inhibition of hydroxyl and strong inhibition of peroxide. FRF at 25 μg mL⁻¹decreased the cellular viability (54.2 ± 0.2 %) using the MTT assay in tumor cell line HepG2 (hepatocellular liver carcinoma cell line). This polysaccharide exhibited anti-angiogenic effect and anticoagulant activity on intrinsic pathway (activated partial thromboplastin time (aPTT). The results showed that FRF from L. variegata has significant pharmacological activity in models of anticoagulation, angiogenesis, and inhibition of free radicals.</description><subject>Acetone</subject><subject>agarose</subject><subject>Algae</subject><subject>angiogenesis</subject><subject>anticoagulant activity</subject><subject>Anticoagulants</subject><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>carcinoma</subject><subject>chemical analysis</subject><subject>Ecology</subject><subject>electrophoresis</subject><subject>Fractionation</subject><subject>free radicals</subject><subject>Freshwater &amp; Marine Ecology</subject><subject>homeostasis</subject><subject>Life Sciences</subject><subject>liver</subject><subject>neoplasm cells</subject><subject>Phaeophycophyta</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Saccharides</subject><subject>thromboplastin</subject><subject>viability</subject><issn>0921-8971</issn><issn>1573-5176</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kUFv1DAQhSMEEkvbH8AJS1zrMuPEcXxEFQWklUBqe7bGjpO6SuPFzrbdf8TPxNtw4MTp2c_vG2v0quo9wgUCqE8ZoUPkgA2HRjQcX1UblKrmElX7utqAFsg7rfBt9S7newDQHXab6vfPuPh5CTQxKsJpHkMc_Rzc-YuxS3EKg0-0hEe_WvE59EWPl_7FcJHG_VROjFyJheXA4lBeQixj2C5Oh0zO3VEKvc_nbNg7mov65yUVwPdsSPGB2RSfZkbTSJ5to427u5iIPRbKj7TQafVmoCn7s796Ut1efbm5_Ma3P75-v_y85a5BWHjb9dJabR3U2lsntGzbulUAUjnprBwQtWg0idYqWQ-is8I2XV8MD96rrj6pPq5zy-a_9j4v5j7u01y-NNgqDai0ViWFa8qlmHPyg9ml8EDpYBDMsRCzFmJKIeZYiMHCiJXJJTuPPv0z-T_QhxUaKBoaU8jm9loASgBRVhS6_gNElJr_</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Castro, Luiza Sheyla Evenni Porfírio Will</creator><creator>de Sousa Pinheiro, Thuane</creator><creator>Castro, Allisson Jhonatan Gomes</creator><creator>da Silva Nascimento Santos, Marilia</creator><creator>Soriano, Eliane Marinho</creator><creator>Leite, Edda Lisboa</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TN</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H95</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20150601</creationdate><title>Potential anti-angiogenic, antiproliferative, antioxidant, and anticoagulant activity of anionic polysaccharides, fucans, extracted from brown algae Lobophora variegata</title><author>Castro, Luiza Sheyla Evenni Porfírio Will ; de Sousa Pinheiro, Thuane ; Castro, Allisson Jhonatan Gomes ; da Silva Nascimento Santos, Marilia ; Soriano, Eliane Marinho ; Leite, Edda Lisboa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acetone</topic><topic>agarose</topic><topic>Algae</topic><topic>angiogenesis</topic><topic>anticoagulant activity</topic><topic>Anticoagulants</topic><topic>antioxidant activity</topic><topic>Antioxidants</topic><topic>Biomedical and Life Sciences</topic><topic>carcinoma</topic><topic>chemical analysis</topic><topic>Ecology</topic><topic>electrophoresis</topic><topic>Fractionation</topic><topic>free radicals</topic><topic>Freshwater &amp; Marine Ecology</topic><topic>homeostasis</topic><topic>Life Sciences</topic><topic>liver</topic><topic>neoplasm cells</topic><topic>Phaeophycophyta</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Saccharides</topic><topic>thromboplastin</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castro, Luiza Sheyla Evenni Porfírio Will</creatorcontrib><creatorcontrib>de Sousa Pinheiro, Thuane</creatorcontrib><creatorcontrib>Castro, Allisson Jhonatan Gomes</creatorcontrib><creatorcontrib>da Silva Nascimento Santos, Marilia</creatorcontrib><creatorcontrib>Soriano, Eliane Marinho</creatorcontrib><creatorcontrib>Leite, Edda Lisboa</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oceanic Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Journal of applied phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castro, Luiza Sheyla Evenni Porfírio Will</au><au>de Sousa Pinheiro, Thuane</au><au>Castro, Allisson Jhonatan Gomes</au><au>da Silva Nascimento Santos, Marilia</au><au>Soriano, Eliane Marinho</au><au>Leite, Edda Lisboa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential anti-angiogenic, antiproliferative, antioxidant, and anticoagulant activity of anionic polysaccharides, fucans, extracted from brown algae Lobophora variegata</atitle><jtitle>Journal of applied phycology</jtitle><stitle>J Appl Phycol</stitle><date>2015-06-01</date><risdate>2015</risdate><volume>27</volume><issue>3</issue><spage>1315</spage><epage>1325</epage><pages>1315-1325</pages><issn>0921-8971</issn><eissn>1573-5176</eissn><abstract>This study analyzed the action of sulfated polysaccharides, fucans, from the brown alga Lobophora variegata on angiogenesis (CAM assays), antioxidant activity, and homeostasis in vitro. Groups of fucans, obtained after acetone fractionation in several (0.3–1.5 v) concentrations, were obtained. This sulfated heterofucan was eluted with 0.8 v of acetone named fraction rich in fucans (FRF). The presence of polysaccharides had its homogeneity confirmed by electrophoresis in agarose with buffer 1,3-diaminopropane and sulfate groups by chemical analyses. We observed that FRF showed antioxidant activity with strong inhibition of hydroxyl and strong inhibition of peroxide. FRF at 25 μg mL⁻¹decreased the cellular viability (54.2 ± 0.2 %) using the MTT assay in tumor cell line HepG2 (hepatocellular liver carcinoma cell line). This polysaccharide exhibited anti-angiogenic effect and anticoagulant activity on intrinsic pathway (activated partial thromboplastin time (aPTT). The results showed that FRF from L. variegata has significant pharmacological activity in models of anticoagulation, angiogenesis, and inhibition of free radicals.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s10811-014-0424-1</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0921-8971
ispartof Journal of applied phycology, 2015-06, Vol.27 (3), p.1315-1325
issn 0921-8971
1573-5176
language eng
recordid cdi_proquest_journals_1679017997
source Springer Link
subjects Acetone
agarose
Algae
angiogenesis
anticoagulant activity
Anticoagulants
antioxidant activity
Antioxidants
Biomedical and Life Sciences
carcinoma
chemical analysis
Ecology
electrophoresis
Fractionation
free radicals
Freshwater & Marine Ecology
homeostasis
Life Sciences
liver
neoplasm cells
Phaeophycophyta
Plant Physiology
Plant Sciences
Saccharides
thromboplastin
viability
title Potential anti-angiogenic, antiproliferative, antioxidant, and anticoagulant activity of anionic polysaccharides, fucans, extracted from brown algae Lobophora variegata
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A08%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Potential%20anti-angiogenic,%20antiproliferative,%20antioxidant,%20and%20anticoagulant%20activity%20of%20anionic%20polysaccharides,%20fucans,%20extracted%20from%20brown%20algae%20Lobophora%20variegata&rft.jtitle=Journal%20of%20applied%20phycology&rft.au=Castro,%20Luiza%20Sheyla%20Evenni%20Porf%C3%ADrio%20Will&rft.date=2015-06-01&rft.volume=27&rft.issue=3&rft.spage=1315&rft.epage=1325&rft.pages=1315-1325&rft.issn=0921-8971&rft.eissn=1573-5176&rft_id=info:doi/10.1007/s10811-014-0424-1&rft_dat=%3Cproquest_cross%3E3676570571%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c410t-68d5bb9bc039ebc295663670057c5cb5f119249a26b753f28b2b48d9a2e0ee783%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1679017997&rft_id=info:pmid/&rfr_iscdi=true