Improved DNA condensation, stability, and transfection with alkyl sulfonyl-functionalized PAMAM G2

In this work, we have used a second-generation PAMAM grafted with octadecyl sulfonyl chains to condense plasmid DNA. The influence of this modification at different levels was investigated by comparison with original PAMAM G2. The condensation process and temporal stability of the complexes was stud...

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Bibliographic Details
Published in:Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2015-04, Vol.17 (4), p.1, Article 198
Main Authors: Rata-Aguilar, Azahara, Maldonado-Valderrama, Julia, Jódar-Reyes, Ana Belén, Ortega-Vinuesa, Juan Luis, Santoyo-Gonzalez, Francisco, Martín-Rodríguez, Antonio
Format: Article
Language:English
Subjects:
Characterization and Evaluation of Materials
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
Inorganic Chemistry
Lasers
Materials Science
Nanoparticles
Nanotechnology
Optical Devices
Optics
Photonics
Physical Chemistry
Research Paper
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Summary:In this work, we have used a second-generation PAMAM grafted with octadecyl sulfonyl chains to condense plasmid DNA. The influence of this modification at different levels was investigated by comparison with original PAMAM G2. The condensation process and temporal stability of the complexes was studied with DLS, finding that the aliphatic chains influence DNA compaction via hydrophobic forces and markedly improve the formation and temporal stability of a single populated system with a hydrodynamic diameter below 100 nm. Interaction with a cell membrane model was also evaluated with a pendant drop tensiometer, resulting in further incorporation of the C18-PAMAM dendriplexes onto the interface. The improvement observed in transfection with our C18 grafted PAMAM is ascribed to the size, stability, and interfacial behavior of the complexes, which in turn are consequence of the DNA condensation process and the interactions involved.
ISSN:1388-0764
1572-896X
DOI:10.1007/s11051-015-3009-2