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Improved DNA condensation, stability, and transfection with alkyl sulfonyl-functionalized PAMAM G2
In this work, we have used a second-generation PAMAM grafted with octadecyl sulfonyl chains to condense plasmid DNA. The influence of this modification at different levels was investigated by comparison with original PAMAM G2. The condensation process and temporal stability of the complexes was stud...
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Published in: | Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2015-04, Vol.17 (4), p.1, Article 198 |
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description | In this work, we have used a second-generation PAMAM grafted with octadecyl sulfonyl chains to condense plasmid DNA. The influence of this modification at different levels was investigated by comparison with original PAMAM G2. The condensation process and temporal stability of the complexes was studied with DLS, finding that the aliphatic chains influence DNA compaction via hydrophobic forces and markedly improve the formation and temporal stability of a single populated system with a hydrodynamic diameter below 100 nm. Interaction with a cell membrane model was also evaluated with a pendant drop tensiometer, resulting in further incorporation of the C18-PAMAM dendriplexes onto the interface. The improvement observed in transfection with our C18 grafted PAMAM is ascribed to the size, stability, and interfacial behavior of the complexes, which in turn are consequence of the DNA condensation process and the interactions involved. |
doi_str_mv | 10.1007/s11051-015-3009-2 |
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The influence of this modification at different levels was investigated by comparison with original PAMAM G2. The condensation process and temporal stability of the complexes was studied with DLS, finding that the aliphatic chains influence DNA compaction via hydrophobic forces and markedly improve the formation and temporal stability of a single populated system with a hydrodynamic diameter below 100 nm. Interaction with a cell membrane model was also evaluated with a pendant drop tensiometer, resulting in further incorporation of the C18-PAMAM dendriplexes onto the interface. 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subjects | Characterization and Evaluation of Materials Chemistry and Materials Science Deoxyribonucleic acid DNA Inorganic Chemistry Lasers Materials Science Nanoparticles Nanotechnology Optical Devices Optics Photonics Physical Chemistry Research Paper |
title | Improved DNA condensation, stability, and transfection with alkyl sulfonyl-functionalized PAMAM G2 |
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