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Evaluation of arboviruses of public health interest in free-living non-human primates (Alouatta spp., Callithrix spp., Sapajus spp.) in Brazil

INTRODUCTION: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso do Sul, Brazil. METHODS: NHP sera samples (to...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2015-03, Vol.48 (2), p.143
Main Authors: Tatiana, Carneiro da Rocha, Paulo, Mira Batista, Andreotti, Renato, Ana, Caroline Dalla Bona, Mário, Antônio Navarro da, Lange, Rogério, Walfrido, Kühl Svoboda, Eliane, Carneiro Gomes
Format: Article
Language:English
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Summary:INTRODUCTION: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso do Sul, Brazil. METHODS: NHP sera samples (total n = 80, Alouatta spp. n = 07, Callithrix spp. n = 29 and Sapajus spp. n = 44) were screened for the presence of viral genomes using reverse transcription polymerase chain reaction and 10% polyacrylamide gel electrophoresis techniques. RESULTS: All of the samples were negative for the Flavivirus genome following the 10% polyacrylamide gel electrophoresis analysis. CONCLUSIONS: These negative results indicate that the analyzed animals were not infected with arboviruses from the Flavivirus genus and did not represent a risk for viral transmission through vectors during the period in which the samples were collected.
ISSN:0037-8682
1678-9849
DOI:10.1590/0037-8682-0024-201