Production of metallo-[beta]-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

INTRODUCTION: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2015-03, Vol.48 (2), p.212
Main Authors: Lívia, Maria do Amorim Costa, Maria, Emília despaceCastro Kling, Geraldo, Renato despace Paula, Lenise, Arneiro Teixeira, Pedro, Juan José Mondino, Sílvia, Susana Bona de Mondino, Cláudia, Rezende despaceVieira Mendonça-Souza
Format: Article
Language:English
Subjects:
Bacteria
Drug resistance
Genetic diversity
Genotype & phenotype
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Summary:INTRODUCTION: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil. METHODS: Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene. CONCLUSIONS: Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.
ISSN:0037-8682
1678-9849
DOI:10.1590/0037-8682-0198-201