Detection and Identification of an Elm Yellows Group Phytoplasma Associated with Camellia in China

In 2012, yellowing of camellias was observed in Tai'an in Shandong province, China. Transmission electron microscopy (TEM) revealed phytoplasma in the phloem sieve tube elements of symptomatic plants. A specific fragment of phytoplasma 16S rRNA gene was amplified by polymerase chain reaction (P...

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Bibliographic Details
Published in:Journal of phytopathology 2015-08, Vol.163 (7-8), p.560-566
Main Authors: Gao, Ying, Dong, Yu‐Zhu, Tan, Wei‐Ping, Sun, Guo‐Zhen, Zhu, You‐Rong, Zhu, Xiao‐Ping
Format: Article
Language:English
Subjects:
16S rRNA gene
16SrV-B subgroup phytoplasma
Camellia
Camellia japonica
Elm yellows phytoplasma
Genes
polymerase chain reaction
restriction fragment length polymorphism
ribosomal RNA
rp gene
sequence analysis
sieve elements
transmission electron microscopy
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Summary:In 2012, yellowing of camellias was observed in Tai'an in Shandong province, China. Transmission electron microscopy (TEM) revealed phytoplasma in the phloem sieve tube elements of symptomatic plants. A specific fragment of phytoplasma 16S rRNA gene was amplified by polymerase chain reaction (PCR) using the universal phytoplasma primers P1/P7 followed by R16F2n/R16R2. Sequence and restriction fragment length polymorphism (RFLP) analyses allowed us to classify the detected phytoplasma into the elm yellows (EY) group (16SrV), subgroup 16SrV‐B. Sequence analyses of the ribosomal protein (rp) gene confirmed a close relationship with phytoplasmas belonging to the rpV‐C subgroup. Thus, the phytoplasma associated with yellows disease in camellia, designated as ‘CY’, is a member of the 16SrV‐B subgroup. This is the first report of phytoplasma associated with camellia.
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.12354