Late Quaternary history of North Eurasian Norway spruce (Picea abies) and Siberian spruce (Picea obovata) inferred from macrofossils, pollen and cytoplasmic DNA variation

• Published in: Journal of biogeography 2015-08, Vol.42 (8), p.1431-1442
• Main Authors: Tollefsrud, Mari Mette, Latałowa, Małgorzata, van der Knaap, W. O., Brochmann, Christian, Sperisen, Christoph
• Format: Article
• Language: English
• Subjects: Deoxyribonucleic acid; DNA; DNA markers; fossil records; Genetic diversity; glacial refugia; introgression; Picea abies; Picea obovata; Pleistocene signals: from Picea to Pseudomisopates; Pollen; post-glacial colonization; Trees
• ISSN: 0305-0270; 1365-2699
• DOI: 10.1111/jbi.12484

Aim: We used combined palaeobotanical and genetic data to assess whether Norway spruce (Picea abies) and Siberian spruce (Picea obovata), two major components of the Eurasian boreal forests, occupied separate glacial refugia, and to test previous hypotheses on their distinction, geographical delimitation and introgression. Location: The range of Norway spruce in northern Europe and Siberian spruce in northern Asia. Methods: Pollen data and recently compiled macrofossil records were summarized for the Last Glacial Maximum (LGM), late glacial and Holocene. Genetic variation was assessed in 50 populations using one maternally (mitochondrial nadl) and one paternally (chloroplast trriT-trnL) inherited marker and analysed using spatial analyses of molecular variance (SAMOVA). Results: Macrofossils showed that spruce was present in both northern Europe and Siberia at the LGM. Congruent macrofossil and pollen data from the late glacial suggested widespread expansions of spruce in the East European Plain, West Siberian Plain, southern Siberian mountains and the Baikal region. Colonization was largely completed during the early Holocene, except in the formerly glaciated area of northern Europe. Both DNA markers distinguished two highly differentiated groups that correspond to Norway spruce and Siberian spruce and coincide spatially with separate LGM spruce occurrences. The division of the mtDNA variation was geographically well defined and occurred to the east of the Ural Mountains along the Ob River, whereas the cpDNA variation showed widespread admixture. Genetic diversity of both DNA markers was higher in western than in eastern populations. Main conclusions: North Eurasian Norway spruce and Siberian spruce are genetically distinct and occupied separate LGM refugia, Norway spruce on the East European Plain and Siberian spruce in southern Siberia, where they were already widespread during the late glacial. They came into contact in the basin of the Ob River and probably hybridized. The lower genetic diversity in the eastern populations may indicate that Siberian spruce suffered more from past climatic fluctuations than Norway spruce.