Detection of Human [alpha]-L-Fucosidases by a Quinone Methide-Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection

[alpha]-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human [alpha]-L-fucosidase in vivo...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2015-07, Vol.16 (11), p.1555
Main Authors: Nandakumar, Manjula, Hsu, Yu-Ling, Lin, Jason C-Y, Lo, Chun, Lo, Lee-Chiang, Lin, Chun-Hung
Format: Article
Language:English
Subjects:
Bacterial infections
Infections
Ulcers
Online Access:Get full text
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Description
Summary:[alpha]-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human [alpha]-L-fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201500178