Effect of cerium oxide nanoparticles on sepsis induced mortality and NF-[kappa]B signaling in cultured macrophages

• Published in: Nanomedicine (London, England) England), 2015-04, Vol.10 (8), p.1275
• Main Authors: Selvaraj, Vellaisamy, Manne, Nandini DPK, Arvapalli, Ravikumar, Rice, Kevin M, Nandyala, Geeta, Fankenhanel, Erin, Blough, Eric R
• Format: Article
• Language: English
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm.14.205

Aim: To investigate whether cerium oxide (CeO2 ) nanoparticles could be used for the treatment of severe sepsis. Materials & methods: Cecal peritonitis was induced in male Sprague-Dawley rats in the presence and absence of CeO2 nanoparticles. Cultured macrophages (RAW264.7 cells) were challenged with lipopolysaccharide in the absence and presence of CeO2 nanoparticles. The effect of nanoparticles on the growth of Escherichia coli and Staphylococcus aureus was determined in culture. Results: Nanoparticle treatment decreased sepsis-induced mortality, organ damage, serum IL-6, blood urea nitrogen and inflammatory markers. Nanoparticle treatment diminished lipopolysaccharide-induced cytokine release and p65-nuclear factor-K B (NF-K B) activation in cultured RAW264.7 cells. Exposure to CeO2 nanoparticles inhibited E. coli growth. Conclusion: The findings of this study indicate that CeO2 nanoparticles may be useful for the treatment of sepsis.