Potential DMSP-degrading Roseobacter clade dominates endosymbiotic microflora of Pyrodinium bahamense var. compressum (Dinophyceae) in vitro

Many aspects of the biology and ecology of the toxic dinoflagellate Pyrodinium bahamense var. compressum are still poorly understood. In this brief note, we present identification of its associated intracellular bacteria or endosymbionts via PCR cloning and 16s rRNA gene sequencing and their localiz...

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Bibliographic Details
Published in:Archives of microbiology 2015-09, Vol.197 (7), p.965-971
Main Authors: Onda, Deo Florence L, Azanza, Rhodora V, Lluisma, Arturo O
Format: Article
Language:English
Subjects:
Algae
Bacteria
bacterial communities
Biochemistry
Biology
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cloning
confocal microscopy
dimethylsulfoniopropionate
Dinoflagellida - microbiology
Dinophyceae
Ecology
endosymbionts
Flavobacteriaceae
genes
Investigations
Life Sciences
Localization
Maximum likelihood method
metabolism
Microbial Ecology
Microbiology
Microscopy
polymerase chain reaction
ribosomal RNA
RNA, Ribosomal, 16S - genetics
Roseobacter
Roseobacter - genetics
Roseobacter - isolation & purification
Roseobacter - metabolism
Roseobacter - physiology
Short Communication
Sulfonium Compounds - metabolism
Symbiosis
toxicity
Water quality
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Summary:Many aspects of the biology and ecology of the toxic dinoflagellate Pyrodinium bahamense var. compressum are still poorly understood. In this brief note, we present identification of its associated intracellular bacteria or endosymbionts via PCR cloning and 16s rRNA gene sequencing and their localization by confocal microscopy, a first for Pyrodinium. The most frequently observed species in the endosymbiotic microflora were from Roseobacter clade (Alphaproteobacteria, 68 %) and Gilvibacter sediminis (Flavobacteriaceae, 20 %). Roseobacter lineage, the most abundant taxa in this study, is known to be involved in dimethylsulfoniopropionate metabolism which is highly produced in dinoflagellates—a possible strong factor shaping the structure of the associated bacterial community.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-015-1133-0