Preparation, Characterization, and Pharmacological Activity of Cymbopogon winterianus Jowitt ex Bor (Poaceae) Leaf Essential Oil of [beta]-Cyclodextrin Inclusion Complexes

• Published in: Evidence-based complementary and alternative medicine 2015-01, Vol.2015
• Main Authors: Santos, Priscila L, Araújo, Adriano A S, Jullyana S. S. Quintans, Oliveira, Makson G B, Brito, Renan G, Serafini, Mairim R, Menezes, Paula P, Santos, Marcio R V, Alves, Pericles B, Waldecy de Lucca Júnior, Blank, Arie F, Viviana La Rocca
• Format: Article
• Language: English
• Subjects: Analgesics; Drugs; Leaves; Natural products; Pharmaceuticals; Proteins; Studies
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2015/502454

This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in β-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in β-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50-200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamate-induced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p