In planta detection used to define the distribution of the European lineages of Phytophthora ramorum on larch (Larix) in the UK

Two genetically distinct evolutionary lineages of the oomycete pathogen Phytophthora ramorum are responsible for the major epidemic on larch (Larix spp.) in the UK: EU1 (historically widespread) and the recently identified EU2 (reported only from Northern Ireland and a small area in southwest Scotla...

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Bibliographic Details
Published in:Plant pathology 2015-10, Vol.64 (5), p.1168-1175
Main Authors: King, K. M, Harris, A. R, Webber, J. F
Format: Article
Language:English
Subjects:
Abies procera
broadleaf
conifer
EU1 lineage
EU2 lineage
Fagus sylvatica
host species
hosts
hybrids
invasive spread
Larix decidua
pathogens
Phytophthora ramorum
plantations
sampling
testing
Tsuga heterophylla
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Summary:Two genetically distinct evolutionary lineages of the oomycete pathogen Phytophthora ramorum are responsible for the major epidemic on larch (Larix spp.) in the UK: EU1 (historically widespread) and the recently identified EU2 (reported only from Northern Ireland and a small area in southwest Scotland). Methods for lineage discrimination have required pure cultures of P. ramorum but, as the pathogen is challenging to isolate from infected larch tissue, only limited data have been available on the distribution of EU2. In this study a protocol was developed to determine the lineage of P. ramorum in infected larch tissue without the need for isolation. The protocol was applied to 134 UK samples collected during 2013–14. In addition, lineage testing was applied to over 300 P. ramorum isolates cultured from a wide range of hosts between 2002 and 2012. Combined data confirmed that EU2 is restricted to Northern Ireland and a small area of southwest Scotland where it is the dominant lineage. There was no evidence of EU2 spread into England and Wales where only EU1 was found. However, EU2 was more widely distributed in southern and eastern parts of Scotland than previously reported. Furthermore, EU1 and EU2 were detected
ISSN:0032-0862
1365-3059
DOI:10.1111/ppa.12345