The shrimp mitochondrial F^sub o^F^sub 1^-ATPase inhibitory factor 1 (IF^sub 1^)

The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial F^sub O^F^sub 1^-ATP synthase is pivotal to the homeosta...

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Bibliographic Details
Published in:Journal of bioenergetics and biomembranes 2015-10, Vol.47 (5), p.383
Main Authors: Chimeo, Cindy, Fernandez-gimenez, Analia Veronica, Campanella, Michelangelo, Mendez-romero, Ofelia, Muhlia-almazan, Adriana
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
ATP
Crustaceans
Dissolved oxygen
Energy sources
Hypoxia
Inhibitor drugs
Marine invertebrates
Mitochondria
Oxygenation
Seawater
Shellfish
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Summary:The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial F^sub O^F^sub 1^-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a F^sub O^F^sub 1^-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the F^sub O^F^sub 1^-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial F^sub O^F^sub 1^-ATPase Inhibitory Factor 1 (IF^sub 1^) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF^sub 1^_Lv1 and the IF^sub 1^_Lv2. The IF^sub 1^_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF^sub 1^_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF^sub 1^ is here discussed as an adaptive mechanism evolved by this marine species to inhibit the F^sub O^F^sub 1^-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension.
ISSN:0145-479X
1573-6881
DOI:10.1007/s10863-015-9621-0