Comparing the Effect of Chlorhexidine and Hydrogen Peroxide on Peri-implantitis Associated Strain of Staphylococcus aureus

• Published in: Journal of international oral health 2015-10, Vol.7 (10), p.13
• Main Authors: Rismanchian, Mansour, Nosouhian, Saeid, Shahabooei, Mohammad, Nourbakhshian, Farzaneh
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteriology; Biofilms; Immunology; Microorganisms; Studies; Titanium; Transplants & implants
• ISSN: 0976-7428; 0976-1799

Staphylococcus aureus is one of the critical microbiotas, which can cause peri-implantitis and endanger dental implants success rate. The aim of this study was to compare the disinfectant properties of chlorhexidine (CHX) and hydrogen peroxide (H2O2) on peri-implantitis associated strain of S. aureus. Totally, 15 implant titanium disks were prepared in the same thickness and diameter. The disks were randomly divided into three groups (n = 5) based on the experimental disinfectants (CHX 0.12% and H2O2 3%) and designated control groups. After the formation of a protein layer on disk surfaces, the specimens were exposed to S. aureus suspension. The decontamination procedure was completed during 5 min for both disinfectants. Trypsin protease 2% was applied to isolate the survived microorganisms at suspension of ½ and ¼. Muller Hinton agar culture was used for microbiota growth. After 48 h incubation, the standard colony forming unit was assayed. Finally, the collected data were analyzed by Kruskal-Wallis and Mann-Whitney tests using SPSS software version 22 at a significant level of 0.05. The Kruskal-Wallis test revealed the significant differences between study groups (P < 0.001). Furthermore, both groups presented significant differences with the control groups (all P < 0.01). Both H2O2 and CHX are effective on S. aureus, nevertheless CHX seemed to be more lethal on studied bacteria but not significantly.