Temperature-responsive Solid-phase Extraction Column for Biological Sample Pretreatment

We have developed a novel solid-phase extraction (SPE) system utilizing a temperature-responsive polymer hydrogelmodified stationary phase. Aminopropyl silica beads (average diameter, 40–64 μm) were coated with poly( N- isopropylacrylamide) (PNIPAAm)-based thermo-responsive hydrogels. Butyl methacry...

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Published in:Analytical sciences 2015, Vol.31 (9), p.881-886
Main Authors: Akimaru, Michiko, Okubo, Kohei, Hiruta, Yuki, Kanazawa, Hideko
Format: Article
Language:English
Subjects:
Acrylic Resins - chemistry
Analytic Sample Preparation Methods - methods
Analytical Chemistry
Chemistry
Humans
Hydrogels - chemistry
Hydrophobic and Hydrophilic Interactions
Phenytoin - isolation & purification
Serum Albumin - isolation & purification
Silicon Dioxide - chemistry
Solid Phase Extraction - methods
Surface Properties
Temperature
Theophylline - isolation & purification
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container_end_page 886
container_issue 9
container_start_page 881
container_title Analytical sciences
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creator Akimaru, Michiko
Okubo, Kohei
Hiruta, Yuki
Kanazawa, Hideko
description We have developed a novel solid-phase extraction (SPE) system utilizing a temperature-responsive polymer hydrogelmodified stationary phase. Aminopropyl silica beads (average diameter, 40–64 μm) were coated with poly( N- isopropylacrylamide) (PNIPAAm)-based thermo-responsive hydrogels. Butyl methacrylate (BMA) and N,N-dimethylaminopropyl acrylamide (DMAPAAm) were used as the hydrophobic and cationic monomers, respectively, and copolymerized with NIPAAm. To evaluate the use of this SPE cartridge for the analysis of drugs and proteins in biological fluids, we studied the separation of phenytoin and theophylline from human serum albumin (HSA) as a model system. The retention of the analytes in an exclusively aqueous eluent could be modulated by changing the temperature and salt content. These results indicated that this temperature-responsive SPE system can be applied to the pretreatment of biological samples for the measurement of serum drug levels.
doi_str_mv 10.2116/analsci.31.881
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source J-STAGE (Japan Science & Tech Info) Free; Springer Nature
subjects Acrylic Resins - chemistry
Analytic Sample Preparation Methods - methods
Analytical Chemistry
Chemistry
Humans
Hydrogels - chemistry
Hydrophobic and Hydrophilic Interactions
Phenytoin - isolation & purification
Serum Albumin - isolation & purification
Silicon Dioxide - chemistry
Solid Phase Extraction - methods
Surface Properties
Temperature
Theophylline - isolation & purification
title Temperature-responsive Solid-phase Extraction Column for Biological Sample Pretreatment
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