effects of osmolality on sperm quality in Jenynsia multidentata (Cyprinodontiformes: Anablepidae)

Sperm quality tests on fish are classically used for evaluating cryopreservation procedures, and they are also promising to assess aquatic toxicity and biomarkers of xenobiotic effects on reproduction. Osmotic shock from the storage medium is one of the main factors affecting sperm quality during ev...

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Published in:Fish physiology and biochemistry 2016-02, Vol.42 (1), p.93-102
Main Authors: da Silva, Janaína Camacho, Varela Junior, Antonio Sergio, Caldas, Jôsie Shwartz, da Silva Freitas, Clarissa, Botelho, Joziel Gonçalves, Colares, Elton Pinto, Corcini, Carine Dahl
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animal Physiology
Animals
Biomarkers
Biomedical and Life Sciences
Cell Membrane
College campuses
Cryopreservation
Cyprinodontiformes - physiology
Deoxyribonucleic acid
DNA
fish
Freshwater & Marine Ecology
Histology
Jenynsia multidentata
Life Sciences
Male
membrane potential
Membrane Potential, Mitochondrial
Membranes
mitochondrial membrane
Morphology
Motility
osmolality
Osmolar Concentration
osmotic stress
plasma membrane
reproduction
semen
Specimen Handling
Sperm
Sperm Motility
spermatozoa
Spermatozoa - physiology
Toxicity
Zoology
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creator da Silva, Janaína Camacho
Varela Junior, Antonio Sergio
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da Silva Freitas, Clarissa
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Colares, Elton Pinto
Corcini, Carine Dahl
description Sperm quality tests on fish are classically used for evaluating cryopreservation procedures, and they are also promising to assess aquatic toxicity and biomarkers of xenobiotic effects on reproduction. Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P 
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Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P &lt; 0.05). The plasma osmolality of J. multidentata is 326 ± 3.9 mOsm/kg. The motility of fresh semen was higher in osmolalities of 280 and 300 mOsm/kg but did not differ between osmolalities from 240 to 320 mOsm/kg. Above 380 mOsm/kg, the motility observed was 0 %. Over the time period studied motility increased with increasing osmolality, and the most constant and long-lasting rates were between 300 and 320 mOsm/kg. On the 4th day of evaluation, higher membrane integrity rates were observed between 280 and 360 mOsm/kg, higher mitochondrial membrane potential was observed between 300 and 460 mOsm/kg, and higher DNA integrity rates were observed between 260 and 380 mOsm/kg. Moreover, osmolalities ≥460 and ≤240 resulted in the lowest motility and DNA integrity levels. Over 4 days, the plasma membrane integrity was significantly lower at ≤260 and ≥400 mOsm/kg, and the mitochondrial membrane potential was significantly lower only in osmolalities ≤240 mOsm/kg. Therefore, we conclude that for sperm quality preservation in J. multidentata, an osmolality of 300–320 mOsm/kg of the most suitable diluent is necessary. 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Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P &lt; 0.05). The plasma osmolality of J. multidentata is 326 ± 3.9 mOsm/kg. The motility of fresh semen was higher in osmolalities of 280 and 300 mOsm/kg but did not differ between osmolalities from 240 to 320 mOsm/kg. Above 380 mOsm/kg, the motility observed was 0 %. Over the time period studied motility increased with increasing osmolality, and the most constant and long-lasting rates were between 300 and 320 mOsm/kg. 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Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P &lt; 0.05). The plasma osmolality of J. multidentata is 326 ± 3.9 mOsm/kg. The motility of fresh semen was higher in osmolalities of 280 and 300 mOsm/kg but did not differ between osmolalities from 240 to 320 mOsm/kg. Above 380 mOsm/kg, the motility observed was 0 %. Over the time period studied motility increased with increasing osmolality, and the most constant and long-lasting rates were between 300 and 320 mOsm/kg. 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Furthermore, we conclude that the storage of sperm in a hyposmotic (&lt;260 mOsm/kg) or hyperosmotic (&gt;400 mOsm/kg) solution affects not only motility but also other sperm quality parameters.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26342693</pmid><doi>10.1007/s10695-015-0120-3</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5683-7801</orcidid></addata></record>
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ispartof Fish physiology and biochemistry, 2016-02, Vol.42 (1), p.93-102
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1573-5168
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subjects Animal Anatomy
Animal Biochemistry
Animal Physiology
Animals
Biomarkers
Biomedical and Life Sciences
Cell Membrane
College campuses
Cryopreservation
Cyprinodontiformes - physiology
Deoxyribonucleic acid
DNA
fish
Freshwater & Marine Ecology
Histology
Jenynsia multidentata
Life Sciences
Male
membrane potential
Membrane Potential, Mitochondrial
Membranes
mitochondrial membrane
Morphology
Motility
osmolality
Osmolar Concentration
osmotic stress
plasma membrane
reproduction
semen
Specimen Handling
Sperm
Sperm Motility
spermatozoa
Spermatozoa - physiology
Toxicity
Zoology
title effects of osmolality on sperm quality in Jenynsia multidentata (Cyprinodontiformes: Anablepidae)
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