Alterations in the Gut Microbiome of Patients with Food Allergy

Methods 12.5 ng of isolated DNA from fecal samples from 29 children with FA (peanut, milk, and/or egg allergy) and 10 healthy, non-atopic controls (C) were amplified using primers specifically for the V3-V4 region of the 16S rRNA gene which is unique to bacteria.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2016-02, Vol.137 (2), p.AB274-AB274
Main Authors: Kiehm, Jamie H., MD, Ponda, Punita, MD, Farzan, Sherry, MD, Weiss, Jared, Sison, Cristina, PhD, Lee, Annette, PhD
Format: Article
Language:English
Subjects:
Allergy and Immunology
Food
Food allergies
Online Access:Get full text
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Description
Summary:Methods 12.5 ng of isolated DNA from fecal samples from 29 children with FA (peanut, milk, and/or egg allergy) and 10 healthy, non-atopic controls (C) were amplified using primers specifically for the V3-V4 region of the 16S rRNA gene which is unique to bacteria.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2015.12.1034