Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross-linking

Here in this paper, xanthine oxidase （XOD） was immobilized onto the chitosan （CHT） modified electrode by a simple way of cross-linking with glutaraldehyde （GTD） and 3-aminopropyltriethoxysilane （KH）. The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and th...

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Bibliographic Details
Published in:Chinese journal of chemistry 2012-07, Vol.30 (7), p.1601-1604
Main Author: 刘玉革 李伟明 魏长宾 吕玲玲
Format: Article
Language:English
Subjects:
chitosan
cross-linking
sensor
xanthine oxidase
交联
修饰电极
制备
壳聚糖
生物传感器
电极传感器
酶的固定化
黄嘌呤氧化酶
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Summary:Here in this paper, xanthine oxidase （XOD） was immobilized onto the chitosan （CHT） modified electrode by a simple way of cross-linking with glutaraldehyde （GTD） and 3-aminopropyltriethoxysilane （KH）. The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 μmol/L with a low detection limit of 0.0215 μmol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxantbine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.201100477