Increased HDL Size and Enhanced Apo A-I Catabolic Rates Are Associated With Doxorubicin-Induced Proteinuria in New Zealand White Rabbits

• Published in: Lipids 2016-03, Vol.51 (3), p.311-320
• Main Authors: López-Olmos, Victoria, Carreón-Torres, Elizabeth, Luna-Luna, María, Flores-Castillo, Cristobal, Martínez-Ramírez, Miriam, Bautista-Pérez, Rocío, Franco, Martha, Sandoval-Zárate, Julio, Roldán, Francisco-Javier, Aranda-Fraustro, Alberto, Soria-Castro, Elizabeth, Muñoz-Vega, Mónica, Fragoso, José-Manuel, Vargas-Alarcón, Gilberto, Pérez-Méndez, Oscar
• Format: Article
• Language: English
• Subjects: Administration, Intravenous; Animals; Apolipoprotein A-I - chemistry; Apolipoprotein A-I - metabolism; Atherosclerosis; Biomedical and Life Sciences; Coronary heart disease; Doxorubicin - administration & dosage; Doxorubicin - adverse effects; HDL; Intravenous administration; Iodine radioisotopes; Life Sciences; Lipidology; Lipids; Lipoprotein metabolism; Lipoproteins, HDL - chemistry; Lipoproteins, HDL - metabolism; Male; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Original Article; Paraoxonase‐1; Particle Size; Proteinuria - chemically induced; Proteinuria - metabolism; Rabbits; Renal failure
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-016-4120-6

The catabolism and structure of high-density lipoproteins (HDL) may be the determining factor of their atheroprotective properties. To better understand the role of the kidney in HDL catabolism, here we characterized HDL subclasses and the catabolic rates of apo A-I in a rabbit model of proteinuria. Proteinuria was induced by intravenous administration of doxorubicin in New Zealand white rabbits ( n  = 10). HDL size and HDL subclass lipids were assessed by electrophoresis of the isolated lipoproteins. The catabolic rate of HDL-apo A-I was evaluated by exogenous radiolabelling with iodine-131. Doxorubicin induced significant proteinuria after 4 weeks (4.47 ± 0.55 vs. 0.30 ± 0.02 g/L of protein in urine, P