Analysis of Helicobacter pylori vacA andcagA genotypes and serum antibody profile in benign and malignant gastroduodenal diseases

• Published in: Gut 1998-08, Vol.43 (2), p.182
• Main Authors: Basso, D, Navaglia, F, Brigato, L, Piva, M G, Toma, A, Greco, E, Di Mario, F, Galeotti, F, Roveroni, G, Corsini, A, Plebani, M
• Format: Article
• Language: English
• Subjects: Antigens; Biomedical research; cagA; Deoxyribonucleic acid; DNA; Endoscopy; gastric adenocarcinoma; Gastric cancer; Genes; Helicobacter pylori; Immunoglobulins; Immunology; Infections; Molecular weight; Polymerase chain reaction; Proteins; Ulcers; vacA; western blot
• ISSN: 0017-5749; 1468-3288
• DOI: 10.1136/gut.43.2.182

Background—Helicobacter pylori species comprise different strains, cytotoxic and non-cytotoxic, which can be identified on the basis of their genomic pattern. Aims—(1) To evaluate the polymorphism of the vacA gene and to ascertain whether thecagA gene is present in patients with gastric adenocarcinoma. (2) To study the anti-H pylori antibody profile using western blotting. Patients—Twenty one patients with gastric adenocarcinoma and 71 with H pyloriassociated benign disease (nine gastric ulcer, 29 duodenal ulcer, 25 antral gastritis, and eight duodenitis). Methods—The polymerase chain reaction was used to verify the presence or absence ofcagA and to study the polymorphism of vacA in gastric mucosal samples obtained during endoscopy for patients with benign diseases and at surgery for patients with gastric adenocarcinoma. Fasting sera were used to assess anti-H pylori antibodies against different H pyloriantigens by western blotting. Results—cagAgene and the allele s1 of vacAwere significantly less frequent in patients with antral gastritis (60% and 60%) compared with patients with gastric adenocarcinoma (94% and 100%) and with other non-malignant gastroduodenal diseases (93% and 87%) (χ2=16.01, p