L-valine, an antialgal amino acid from Streptomyces jiujiangensis JXJ 0074^sup T

An antialgal compound was isolated from the cultured broth of Streptomyces jiujiangensis JXJ 0074^sup T^ by using bioassay methods. Based on the data of ^sup 1^H-NMR, ^sup 13^C-NMR, ESI-MS, and thin layer chromatography, the active compound was identified as L-valine, which showed antialgal activity...

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Published in:Applied microbiology and biotechnology 2016-05, Vol.100 (10), p.4627
Main Authors: Zhang, Bing-huo, Chen, Wei, Li, Han-quan, Yang, Jian-yuan, Zha, Dai-ming, Duan, Yan-qing, Nhozzein, Wael, Xiao, Min, Gao, Rui, Li, Wen-jun
Format: Article
Language:English
Subjects:
Algae
Amino acids
Analysis
Antimicrobial agents
Bioassays
Biosynthesis
Biotechnology
Cellular biology
Chlorophyll
Cyanobacteria
Laboratories
Microcystins
Pallets
Peroxidation
Studies
Thin layer chromatography
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Summary:An antialgal compound was isolated from the cultured broth of Streptomyces jiujiangensis JXJ 0074^sup T^ by using bioassay methods. Based on the data of ^sup 1^H-NMR, ^sup 13^C-NMR, ESI-MS, and thin layer chromatography, the active compound was identified as L-valine, which showed antialgal activity mainly against Microcystis. L-valine exhibited greater antialgal activities than both L-lysine and copper sulfate (CuSO^sub 4^) did on Microcystis aeruginosa lawn. However, M. aeruginosa recovered growth earlier with higher growth rate in L-valine treatment than in L-lysine treatment. L-valine dissipated completely within 2 days, much quicker than L-lysine (6 days), which resulted in the lysing of more than 80 % M. aeruginosa cells and the release of amount of intracellular microcystin-LR (MC-LR) within 2 days. As a resultant, the extracellular MC-LR content was more than twice of the control from day 1 to 5. Exposure to L-valine significantly promoted the synthesis of MC-LR. L-lysine also promoted the release and synthesis of MC-LR with much lesser efficiency than L-valine. L-valine could damage Microcystis severely, causing perforation and collapse of M. aeruginosa cells and decrease of the chlorophyll. The superoxide dismutase (SOD) activity in L-valine-treated cells of M. aeruginosa initially increased with 32.94±3.37 % higher than the control after 36 h and then decreased quickly. However, the increase rate of superoxide anion radical (O2 ^sup -^) was much higher than that of SOD, which resulted in serious lipid peroxidation and accumulation of malondialdehyde (MDA). To our knowledge, this is the first report showing L-valine active against cyanobacteria.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-015-7150-8