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Preparation of resistant starch by dual modified method from indica rice starch
Resistant starch (RS) is defined as the part of the total starch that resists hydrolysis and absorption in the small intestine. RS has been proved to be beneficial to human health. Biochemical modification is also an effective way to prepare RS. In this study, we prepared RS from Indica rice starch...
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Published in: | Current topics in nutraceuticals research 2015-11, Vol.13 (4), p.173 |
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description | Resistant starch (RS) is defined as the part of the total starch that resists hydrolysis and absorption in the small intestine. RS has been proved to be beneficial to human health. Biochemical modification is also an effective way to prepare RS. In this study, we prepared RS from Indica rice starch using thermal treatment and dual-enzymatic (α-amylase and pullulanase) modification. Hydrolysis by increasing concentrations of α-amylase (0, 2, 4, 6, 8 and 10 U×g-1) was evaluated at increasing temperatures from 50 to 100°C, and pH between 4.5 and 7.0 for 10 to 60 min. The optimal conditions for hydrolysis was 6 U×g-1 α-amylase at 80 °C for 40 min with pH adjusted to 5.5. Under these conditions, the RS yield was 45.3%. Moreover, the RS product obtained under these conditions exhibited a lower hydrolysis rate than other starch samples. |
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RS has been proved to be beneficial to human health. Biochemical modification is also an effective way to prepare RS. In this study, we prepared RS from Indica rice starch using thermal treatment and dual-enzymatic (α-amylase and pullulanase) modification. Hydrolysis by increasing concentrations of α-amylase (0, 2, 4, 6, 8 and 10 U×g-1) was evaluated at increasing temperatures from 50 to 100°C, and pH between 4.5 and 7.0 for 10 to 60 min. The optimal conditions for hydrolysis was 6 U×g-1 α-amylase at 80 °C for 40 min with pH adjusted to 5.5. Under these conditions, the RS yield was 45.3%. 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RS has been proved to be beneficial to human health. Biochemical modification is also an effective way to prepare RS. In this study, we prepared RS from Indica rice starch using thermal treatment and dual-enzymatic (α-amylase and pullulanase) modification. Hydrolysis by increasing concentrations of α-amylase (0, 2, 4, 6, 8 and 10 U×g-1) was evaluated at increasing temperatures from 50 to 100°C, and pH between 4.5 and 7.0 for 10 to 60 min. The optimal conditions for hydrolysis was 6 U×g-1 α-amylase at 80 °C for 40 min with pH adjusted to 5.5. Under these conditions, the RS yield was 45.3%. Moreover, the RS product obtained under these conditions exhibited a lower hydrolysis rate than other starch samples.</description><subject>Binding sites</subject><subject>Hydrogen bonds</subject><subject>Methods</subject><subject>Nutrient interactions</subject><subject>Nutritional aspects</subject><subject>Physiological aspects</subject><subject>Properties</subject><subject>Rice</subject><subject>Starch</subject><subject>Studies</subject><issn>1540-7535</issn><issn>2641-452X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNptkEtLxDAQx4MoWFe_Q0DwVknTpEmOy-ILFtaDgreS5rHN0jZrkh789kbcQwUZmBmG3_zncQYK3JCqJBR_nIOiogSVjNb0ElzFeECoqTHHBdi9BnOUQSbnJ-gtDCa6mOSUYPZB9bD7gnqWAxy9dtYZDUeTeq-hDX6EbtJOSRicMif-GlxYOURzc4or8P748LZ5Lre7p5fNelvuMa9SKYyQtkOkYlYS01BEtBYVY0grSxvS4E7VXCClFWJMctRoknfPBylJO57zFbj91T0G_zmbmNqDn8OUR7YV41RkDbqg9nIwrZusT0Gq0UXVrgkVWFDCSabu_6GyaTM65SdjXa7_abhbNPRGDqmPfph_nhiX4DcQg3PX</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Wang, Wen-Juan</creator><creator>Yuan, Huai-Bo</creator><creator>Chen, De-Yi</creator><creator>Meng, Shao-Hua</creator><creator>Zhu, Xi-Ping</creator><general>New Century Health Publishers, LLC</general><scope>3V.</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PADUT</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20151101</creationdate><title>Preparation of resistant starch by dual modified method from indica rice starch</title><author>Wang, Wen-Juan ; Yuan, Huai-Bo ; Chen, De-Yi ; Meng, Shao-Hua ; Zhu, Xi-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g281t-9e9afb0417fa4e6504dd91770dcf56462bc3890cdc077a806d4535264ca5b8453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Binding sites</topic><topic>Hydrogen bonds</topic><topic>Methods</topic><topic>Nutrient interactions</topic><topic>Nutritional aspects</topic><topic>Physiological aspects</topic><topic>Properties</topic><topic>Rice</topic><topic>Starch</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Wen-Juan</creatorcontrib><creatorcontrib>Yuan, Huai-Bo</creatorcontrib><creatorcontrib>Chen, De-Yi</creatorcontrib><creatorcontrib>Meng, Shao-Hua</creatorcontrib><creatorcontrib>Zhu, Xi-Ping</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Research Library</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Research Library China</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Current topics in nutraceuticals research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Wen-Juan</au><au>Yuan, Huai-Bo</au><au>Chen, De-Yi</au><au>Meng, Shao-Hua</au><au>Zhu, Xi-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of resistant starch by dual modified method from indica rice starch</atitle><jtitle>Current topics in nutraceuticals research</jtitle><date>2015-11-01</date><risdate>2015</risdate><volume>13</volume><issue>4</issue><spage>173</spage><pages>173-</pages><issn>1540-7535</issn><eissn>2641-452X</eissn><abstract>Resistant starch (RS) is defined as the part of the total starch that resists hydrolysis and absorption in the small intestine. RS has been proved to be beneficial to human health. Biochemical modification is also an effective way to prepare RS. In this study, we prepared RS from Indica rice starch using thermal treatment and dual-enzymatic (α-amylase and pullulanase) modification. Hydrolysis by increasing concentrations of α-amylase (0, 2, 4, 6, 8 and 10 U×g-1) was evaluated at increasing temperatures from 50 to 100°C, and pH between 4.5 and 7.0 for 10 to 60 min. The optimal conditions for hydrolysis was 6 U×g-1 α-amylase at 80 °C for 40 min with pH adjusted to 5.5. Under these conditions, the RS yield was 45.3%. Moreover, the RS product obtained under these conditions exhibited a lower hydrolysis rate than other starch samples.</abstract><cop>Coppell</cop><pub>New Century Health Publishers, LLC</pub></addata></record> |
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subjects | Binding sites Hydrogen bonds Methods Nutrient interactions Nutritional aspects Physiological aspects Properties Rice Starch Studies |
title | Preparation of resistant starch by dual modified method from indica rice starch |
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