Long non-coding RNA CASC11 interacts with hnRNP-K and activates the WNT/[beta]-catenin pathway to promote growth and metastasis in colorectal cancer

• Published in: Cancer letters 2016-06, Vol.376 (1), p.62
• Main Authors: Zhang, Zheying, Zhou, Chang, Chang, Yaya, Zhang, Zuoyang, Hu, Yuhan, Zhang, Fan, Lu, Yanxia, Zheng, Lin, Zhang, Wenjuan, Li, Xiaomin, Li, Xuenong
• Format: Article
• Language: English
• Subjects: Animal sciences; Cell growth; Colorectal cancer; Genes; Laboratories; Metastasis; Plasmids; Prostate cancer; RNA polymerase; Rodents; Zoology
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2016.03.022

The abnormal expression of many long non-coding RNAs (lncRNAs) has been reported in the progression of various tumors, and these lncRNAs can be useful as diagnostic indicators and anti-tumor targets. Therefore, it is important to identify lncRNAs that can be used for the clinical prevention and treatment of colorectal cancer (CRC). Here, we report that cancer susceptibility candidate 11 (CASC11) was upregulated in CRC tissues; increased CASC11 expression in CRC was associated with tumor size, serosal invasion, lymph metastasis, and the tumor-node-metastasis (TNM) stage. Functional experiments showed that CASC11 can promote CRC cell proliferation and metastasis in vitro and in vivo. Furthermore, CASC11 can target heterogeneous ribonucleoprotein K (hnRNP-K) to activate WNT/β-catenin signaling in CRC cells. In addition, we found that c-Myc directly bound to the promoter regions of CASC11 and increased promoter histone acetylation to enhance CASC11 expression. Together, our findings indicate that the novel lncRNA CASC11 may serve as a candidate diagnostic biomarker and a promising therapeutic target for CRC.