Estimating the DNA strand breakage using a fuzzy inference system and agarose gel electrophoresis, a case study with toothed carp Aphanius sophiae exposed to cypermethrin

The DNA breakage has been widely used in ecotoxicological studies to investigate effects of pesticides in fishes. The present study used a fuzzy inference system to quantify the breakage of DNA double strand in Aphanius sophiae exposed to the cypermethrin. The specimens were adapted to different tem...

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Bibliographic Details
Published in:Ecotoxicology (London) 2016-07, Vol.25 (5), p.1040-1046
Main Authors: Poorbagher, Hadi, Moghaddam, Maryam Nasrollahpour, Eagderi, Soheil, Farahmand, Hamid
Format: Article
Language:English
Subjects:
Animals
Carps - physiology
Case studies
Cypermethrin
Deoxyribonucleic acid
DNA
DNA Damage
Earth and Environmental Science
Ecology
Ecotoxicology
Electrophoresis, Agar Gel
Environment
Environmental Management
Ethylenediaminetetraacetic acid
Fuzzy Logic
Gills
Insecticides - toxicity
Mutagenicity Tests - methods
Pesticides
Pyrethrins - toxicity
Salinity
Surface water
Technical Note
Variance analysis
Water Pollutants, Chemical - toxicity
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Summary:The DNA breakage has been widely used in ecotoxicological studies to investigate effects of pesticides in fishes. The present study used a fuzzy inference system to quantify the breakage of DNA double strand in Aphanius sophiae exposed to the cypermethrin. The specimens were adapted to different temperatures and salinity for 14 days and then exposed to cypermethrin. DNA of each specimens were extracted, electrophoresed and photographed. A fuzzy system with three input variables and 27 rules were defined. The pixel value curve of DNA on each gel lane was obtained using ImageJ. The DNA breakage was quantified using the pixel value curve and fuzzy system. The defuzzified values were analyzed using a three-way analysis of variance. Cypermethrin had significant effects on DNA breakage. Fuzzy inference systems can be used as a tool to quantify the breakage of double strand DNA. DNA double strand of the gill of A. sophiae is sensitive enough to be used to detect cypermethrin in surface waters in concentrations much lower than those reported in previous studies.
ISSN:0963-9292
1573-3017
DOI:10.1007/s10646-016-1647-5