Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/[beta]-catenin signaling pathway

HSPA1A, which encodes cognate heat shock protein 70, plays important roles in various cellular metabolic pathways. To investigate its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), its expression level was compared between undifferentiated and differentiated BMS...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-06, Vol.6, p.27622
Main Authors: Zhang, Wei, Xue, Deting, Yin, Houfa, Wang, Shengdong, Li, Chao, Chen, Erman, Hu, Dongcai, Tao, Yiqing, Yu, Jiawei, Zheng, Qiang, Gao, Xiang, Pan, Zhijun
Format: Article
Language:English
Subjects:
Biotechnology
Bone marrow
Fractures
Heat
Heat shock proteins
Penicillin
Stem cells
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:HSPA1A, which encodes cognate heat shock protein 70, plays important roles in various cellular metabolic pathways. To investigate its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), its expression level was compared between undifferentiated and differentiated BMSCs. Rat HSPA1A overexpression in BMSCs increased osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Moreover, it upregulated β-catenin and downregulated DKK1 and SOST. The enhanced osteogenesis due to HSPA1A overexpression was partly rescued by a Wnt/β-catenin inhibitor. Additionally, using a rat tibial fracture model, a sheet of HSPA1A-overexpressing BMSCs improved bone fracture healing, as determined by imaging and histological analysis. Taken together, these findings suggest that HSPA1A overexpression enhances osteogenic differentiation of BMSCs, partly through Wnt/β-catenin.
ISSN:2045-2322
DOI:10.1038/srep27622