Optimization of Antioxidant Activity and Phenolic Compound Extraction Conditions from Red Seaweed (Laurencia obtuse)

• Published in: Journal of aquatic food product technology 2016-04, Vol.25 (3), p.414-422
• Main Authors: Topuz, Osman Kadir, Gokoglu, Nalan, Yerlikaya, Pinar, Ucak, Ilknur, Gumus, Bahar
• Format: Article
• Language: English
• Subjects: Algae; Antioxidants; Laurencia; Laurencia obtuse; phenolic compound extraction; Phenols; Red seaweed; response surface methodology; Solvents; Temperature
• ISSN: 1049-8850; 1547-0636
• DOI: 10.1080/10498850.2013.868844

Response surface methodology was used to investigate the effect of ultrasound-assisted extraction conditions including solvent:seaweed ratio (10:1-30:1), extraction temperature (30-50°C), and extraction time (30-60 min) on the total phenolic compounds and antioxidant activity of red seaweed (Laurencia obtuse) extracts. The optimum extraction parameters for maximum phenolic content were as follows: solvent:seaweed ratio, 30:1; extraction temperature, 50°C; and extraction time, 42.8 min. The experiment results showed that the solvent:seaweed ratio was the most significant parameter for the extraction. The optimum extraction conditions for maximum antioxidant activity were as follows: solvent:seaweed ratio, 24.3:1; extraction temperature, 45.3°C; and extraction time, 58 min. Under the above-mentioned conditions, the experimental total phenolic content and antioxidant activity value were 26.23 ± 0.75 mg GAE/g seaweed and 120.89 ± 0.81 TEAC, respectively, of seaweed tested, which are well compatible with the predicted contents.