Effective and Low-Cost Saccharification of Pineapple Peel by Trichoderma viride Crude Extract with Enhanced [beta]-Glucosidase Activity

Efficient, low-cost enzymatic hydrolysis of lignocellulosic biomass is essential for cost-effective production of bioethanol. The aim of this study was to establish a fungal fermentation-based strategy for the economic enzymatic conversion of pineapple peel into fermentable sugars. Trichoderma virid...

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Bibliographic Details
Published in:Bioenergy research 2016-09, Vol.9 (3), p.701
Main Authors: de Almeida, Janaína Marques, de Lima, Vanderlei Aparecido, de Lima, Patrícia Carla Giloni, Knob, Adriana
Format: Article
Language:English
Subjects:
Agricultural wastes
Biodiesel fuels
Biomass
Cellulase
Cellulose
Cultivation
Economic aspects
Enzymes
Ethanol
Fermentation
Fruits
Glucose
Hydrolases
Hydrolysis
Industrial wastes
Lignocellulose
Organic waste treatment
Raw materials
Regression analysis
Sugar
Waste materials
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Summary:Efficient, low-cost enzymatic hydrolysis of lignocellulosic biomass is essential for cost-effective production of bioethanol. The aim of this study was to establish a fungal fermentation-based strategy for the economic enzymatic conversion of pineapple peel into fermentable sugars. Trichoderma viride was grown on passion fruit peel in order to improve its [beta]-glucosidase production, and a crude extract was then used to hydrolyze pineapple peel. The effects of medium pH, cultivation time, and passion fruit peel concentration on [beta]-glucosidase production were evaluated using a central composite rotational design (CCRD) combined with response surface methodology (RSM). Optimal [beta]-glucosidase activity of 2.40 U mL.sup.-1 was found after 6.5 days of cultivation in medium at pH 6.0, containing 2.0 % passion fruit peel. Saccharification of pineapple peel was also optimized by RSM and CCRD with respect to pH, temperature, [beta]-glucosidase concentration, and reaction time and proceeded optimally at pH 4.0, 55 °C, with a [beta]-glucosidase loading of 31.25 U g.sup.-1 dry feedstock and 75 h of reaction. Under these conditions, T. viride crude extract hydrolyzed pineapple peel with a glucose yield of 65.3 %. This study therefore presents passion fruit peel as an attractive raw material for the production of [beta]-glucosidases. In addition, it describes an improved, effective, and low-cost enzymatic method for the production of fermentable sugars from pineapple peel, an abundant and inexpensive agro-industrial waste.
ISSN:1939-1234
1939-1242
DOI:10.1007/s12155-016-9714-6