Redox-dependent ferric oxide nanoparticles loaded with doxorubicin and their influence on the functions of mitochondria

• Published in: Biophysics (Oxford) 2014-09, Vol.59 (5), p.732-735
• Main Authors: Fedotcheva, T. A., Akopdjanov, A. G., Shimanovskii, N. L., Mingalev, V. V., Banin, V. V., Zemlanaya, A. A., Teplova, V. V., Fedotcheva, N. I.
• Format: Article
• Language: English
• Subjects: Antioxidants; Biological and Medical Physics; Biophysics; Cell Biophysics; Fluorescence; Mitochondria; Nanoparticles; Physics; Physics and Astronomy
• ISSN: 0006-3509; 1555-6654
• DOI: 10.1134/S0006350914050078

The spectral, fluorescent and functional properties of ferric oxide and ferric hydroxide nanoparticles loaded with doxorubicin and stabilized with citric acid or lysine were studied in comparison with free doxorubicin. Their effect on the opening of calcium-induced mitochondrial pore and the possibility of controlled release of doxorubicin under the influence of redox stimuli were investigated. The data show that the effect of nanoparticles on mitochondria depends on the type of stabilizer. The spectral and fluorescence methods used allow us to estimate the presence or absence of free doxorubicin in solution of nanoparticles and the concentration of bound doxorubicin. It is shown that dithiothreitol and glutathione increase the amplitude of absorption and fluorescence of doxorubicin during incubation with nanoparticles. It is assumed that this effect may be associated with the reduction of oxidized iron by thiols with subsequent release of doxorubicin.