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Survival and Fertility of Antibiotic-Treated Bovine Spermatozoa

Motility of spermatozoa stored at 5C with up to 1000 units or μg of chloramphenicol, polymyxin, kanamycin, tylosin, ampicillin, lincomycin, spectinomycin, erythromycin, novabiocin, or terramycin per ml of extender was compared to that with penicillin plus dihydrostreptomycin. Novabiocin and terramyc...

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Published in:Journal of dairy science 1976-12, Vol.59 (12), p.2130-2133
Main Authors: Berndtson, W.E., Foote, R.H.
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Language:English
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description Motility of spermatozoa stored at 5C with up to 1000 units or μg of chloramphenicol, polymyxin, kanamycin, tylosin, ampicillin, lincomycin, spectinomycin, erythromycin, novabiocin, or terramycin per ml of extender was compared to that with penicillin plus dihydrostreptomycin. Novabiocin and terramycin were toxic, but other antibiotic treatments had no effect. However, erythromycin and tylosin, as well as colymycin, depressed motility of frozen thawed spermatozoa. Spermatozoal motility was equivalent, following freezing in ampules or straws. All of the antibiotics which were nontoxic when added singly to frozen semen were also not harmful to frozen spermatozoa when as much as 2000 units or μg were added per ml of extender containing penicillin and dihydrostreptomycin. The addition of 1000 units or μg of ampicillin, chloramphenicol, kanamycin, lincomycin, polymyxin, or spectinomycin per ml of extender containing 750 units penicillin and 750μg dihydrostreptomycin per ml did not influence the fertility of frozen spermatozoa in a field test involving 19,663 first inseminations.
doi_str_mv 10.3168/jds.S0022-0302(76)84498-0
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subjects Animals
Anti-Bacterial Agents - pharmacology
Cattle - physiology
Cell Survival
Citrates - pharmacology
Colistin - pharmacology
Dihydrostreptomycin Sulfate - pharmacology
Egg Yolk
Erythromycin - pharmacology
Female
Leucomycins - pharmacology
Male
Oxytetracycline - pharmacology
Penicillins - pharmacology
Sperm Motility - drug effects
Spermatozoa - drug effects
Tromethamine - pharmacology
title Survival and Fertility of Antibiotic-Treated Bovine Spermatozoa
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