Isolation and characterization of microsatellite DNA loci from Sillago sihama

The silver sillago, Sillago sihama Forsskl is naturally distributed in IndoWest Pacific: Red Sea and Knysna, South Africa to Japan and south to Australia, and is common along beaches, sandbars, mangrove creeks and estuaries. Adults bury themselves in the sand when disturbed, and feed mainly on polyc...

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Bibliographic Details
Published in:Journal of genetics 2014-12, Vol.93 (Suppl 1), p.32-36
Main Authors: GUO, YU-SONG, WANG, ZHONG-DUO, YAN, CHENG-ZHONG, ZHANG, YU-LAN, ZHENG, JIN-NAN, XU, YUAN-MIN, DU, TAO, LIU, CHU-WU
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biomedical and Life Sciences
Evolutionary Biology
Fish
Gene loci
Genetic diversity
Genetic markers
Genetic research
Life Sciences
Microbial Genetics and Genomics
Online Resources
Plant Genetics and Genomics
Satellite DNA
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Summary:The silver sillago, Sillago sihama Forsskl is naturally distributed in IndoWest Pacific: Red Sea and Knysna, South Africa to Japan and south to Australia, and is common along beaches, sandbars, mangrove creeks and estuaries. Adults bury themselves in the sand when disturbed, and feed mainly on polychaete worms, small prawns (Penaeus), shrimps and amphipods. This fish is found in all Chinese waters including East China Sea, Yellow Sea, Bohai Sea, South China Sea, and the Northern Bay. In the present study, we developed 66 microsatellite loci isolated from a dinucleotide-enriched genomic library of S. sihama using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol of Zane et al. (2002). These markers should provide a tool for assessing genetic diversity and population structure of wild stocks.
ISSN:0022-1333
0973-7731
DOI:10.1007/s12041-012-0133-y