Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period

Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynapti...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2016-10, Vol.10 (4), p.338-340
Main Authors: Sibarov, D. A., Abushik, P. A., Antonov, S. M.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell Biology
Extracellular matrix
Life Sciences
Magnesium
Neurons
Short Communications
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c268t-83773df275cb6aab988ddba0b083d5c1f4d6a6d0af58b845cd7b50743d046c5b3
container_end_page 340
container_issue 4
container_start_page 338
container_title Biochemistry (Moscow). Supplement series A, Membrane and cell biology
container_volume 10
creator Sibarov, D. A.
Abushik, P. A.
Antonov, S. M.
description Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in EC. Ifenprodil did not block EC but strongly inhibited NMDA-evoked whole-cell currents, which say for a key role of the ifenprodil-resistant synaptic GluN2A-containing NMDA receptors in the generation of EC. We suppose that in cultured neurons the onset of EC and a gradual increase of the EC amplitude over the time of culture period is directed by an increase of synaptic connections density and displacement of the GluN2B subunit by GluN2A in synapses.
doi_str_mv 10.1134/S1990747816040073
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1848411646</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4277234811</sourcerecordid><originalsourceid>FETCH-LOGICAL-c268t-83773df275cb6aab988ddba0b083d5c1f4d6a6d0af58b845cd7b50743d046c5b3</originalsourceid><addsrcrecordid>eNp1kEFLxDAQhYMouK7-AG8Bz9VkkybpUZbVFRY8qFdLmqSapdvUSbrgvzdlRQTxNI_H-94wg9AlJdeUMn7zRKuKSC4VFYQTItkRmk1WIXnFj3-0VKfoLMYtIYJxIWbodTX4zg3JtwF22Izg-hSx7zHohE2A5I3ucO9GCP3kG3A6Ohz2DnDyu6xanN7dROx9gpArupRb8ODAB3uOTlrdRXfxPefo5W71vFwXm8f7h-XtpjALoVKhmJTMtgtZmkZo3VRKWdto0hDFbGloy63QwhLdlqpRvDRWNmU-l1nChSkbNkdXh94BwsfoYqq3YYQ-r6yp4opTKrjIKXpIGQgxgmvrAfxOw2dNST29sf7zxswsDkzM2f7Nwa_mf6EvTRR1dQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1848411646</pqid></control><display><type>article</type><title>Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period</title><source>Springer Nature</source><creator>Sibarov, D. A. ; Abushik, P. A. ; Antonov, S. M.</creator><creatorcontrib>Sibarov, D. A. ; Abushik, P. A. ; Antonov, S. M.</creatorcontrib><description>Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in EC. Ifenprodil did not block EC but strongly inhibited NMDA-evoked whole-cell currents, which say for a key role of the ifenprodil-resistant synaptic GluN2A-containing NMDA receptors in the generation of EC. We suppose that in cultured neurons the onset of EC and a gradual increase of the EC amplitude over the time of culture period is directed by an increase of synaptic connections density and displacement of the GluN2B subunit by GluN2A in synapses.</description><identifier>ISSN: 1990-7478</identifier><identifier>EISSN: 1990-7494</identifier><identifier>DOI: 10.1134/S1990747816040073</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; Extracellular matrix ; Life Sciences ; Magnesium ; Neurons ; Short Communications</subject><ispartof>Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2016-10, Vol.10 (4), p.338-340</ispartof><rights>Pleiades Publishing, Ltd. 2016</rights><rights>Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology is a copyright of Springer, 2016.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-83773df275cb6aab988ddba0b083d5c1f4d6a6d0af58b845cd7b50743d046c5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sibarov, D. A.</creatorcontrib><creatorcontrib>Abushik, P. A.</creatorcontrib><creatorcontrib>Antonov, S. M.</creatorcontrib><title>Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period</title><title>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</title><addtitle>Biochem. Moscow Suppl. Ser. A</addtitle><description>Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in EC. Ifenprodil did not block EC but strongly inhibited NMDA-evoked whole-cell currents, which say for a key role of the ifenprodil-resistant synaptic GluN2A-containing NMDA receptors in the generation of EC. We suppose that in cultured neurons the onset of EC and a gradual increase of the EC amplitude over the time of culture period is directed by an increase of synaptic connections density and displacement of the GluN2B subunit by GluN2A in synapses.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Extracellular matrix</subject><subject>Life Sciences</subject><subject>Magnesium</subject><subject>Neurons</subject><subject>Short Communications</subject><issn>1990-7478</issn><issn>1990-7494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kEFLxDAQhYMouK7-AG8Bz9VkkybpUZbVFRY8qFdLmqSapdvUSbrgvzdlRQTxNI_H-94wg9AlJdeUMn7zRKuKSC4VFYQTItkRmk1WIXnFj3-0VKfoLMYtIYJxIWbodTX4zg3JtwF22Izg-hSx7zHohE2A5I3ucO9GCP3kG3A6Ohz2DnDyu6xanN7dROx9gpArupRb8ODAB3uOTlrdRXfxPefo5W71vFwXm8f7h-XtpjALoVKhmJTMtgtZmkZo3VRKWdto0hDFbGloy63QwhLdlqpRvDRWNmU-l1nChSkbNkdXh94BwsfoYqq3YYQ-r6yp4opTKrjIKXpIGQgxgmvrAfxOw2dNST29sf7zxswsDkzM2f7Nwa_mf6EvTRR1dQ</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Sibarov, D. A.</creator><creator>Abushik, P. A.</creator><creator>Antonov, S. M.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20161001</creationdate><title>Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period</title><author>Sibarov, D. A. ; Abushik, P. A. ; Antonov, S. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-83773df275cb6aab988ddba0b083d5c1f4d6a6d0af58b845cd7b50743d046c5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Extracellular matrix</topic><topic>Life Sciences</topic><topic>Magnesium</topic><topic>Neurons</topic><topic>Short Communications</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sibarov, D. A.</creatorcontrib><creatorcontrib>Abushik, P. A.</creatorcontrib><creatorcontrib>Antonov, S. M.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Materials science collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sibarov, D. A.</au><au>Abushik, P. A.</au><au>Antonov, S. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period</atitle><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle><stitle>Biochem. Moscow Suppl. Ser. A</stitle><date>2016-10-01</date><risdate>2016</risdate><volume>10</volume><issue>4</issue><spage>338</spage><epage>340</epage><pages>338-340</pages><issn>1990-7478</issn><eissn>1990-7494</eissn><abstract>Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in EC. Ifenprodil did not block EC but strongly inhibited NMDA-evoked whole-cell currents, which say for a key role of the ifenprodil-resistant synaptic GluN2A-containing NMDA receptors in the generation of EC. We suppose that in cultured neurons the onset of EC and a gradual increase of the EC amplitude over the time of culture period is directed by an increase of synaptic connections density and displacement of the GluN2B subunit by GluN2A in synapses.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1990747816040073</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1990-7478
ispartof Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2016-10, Vol.10 (4), p.338-340
issn 1990-7478
1990-7494
language eng
recordid cdi_proquest_journals_1848411646
source Springer Nature
subjects Biochemistry
Biomedical and Life Sciences
Cell Biology
Extracellular matrix
Life Sciences
Magnesium
Neurons
Short Communications
title Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T05%3A43%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Epileptiform%20curents%20in%20rat%20cortical%20neurons%20increase%20over%20time%20of%20the%20in%20vitro%20culture%20period&rft.jtitle=Biochemistry%20(Moscow).%20Supplement%20series%20A,%20Membrane%20and%20cell%20biology&rft.au=Sibarov,%20D.%20A.&rft.date=2016-10-01&rft.volume=10&rft.issue=4&rft.spage=338&rft.epage=340&rft.pages=338-340&rft.issn=1990-7478&rft.eissn=1990-7494&rft_id=info:doi/10.1134/S1990747816040073&rft_dat=%3Cproquest_cross%3E4277234811%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c268t-83773df275cb6aab988ddba0b083d5c1f4d6a6d0af58b845cd7b50743d046c5b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1848411646&rft_id=info:pmid/&rfr_iscdi=true