Complete genome sequence of Vibrio parahaemolyticus FORC_023 isolated from raw fish storage water

Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that causes food-borne gastroenteritis in humans who consume V. parahaemolyticus-contaminated seafood. The FORC_023 strain was isolated from raw fish storage water, containing live fish at a sashimi restaurant. Here, we aimed to sequenc...

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Published in:Pathogens and disease 2016-06, Vol.74 (4), p.ftw032
Main Authors: Chung, Han Young, Na, Eun Jung, Lee, Kyu-Ho, Ryu, Sangryeol, Yoon, Hyunjin, Lee, Ju-Hoon, Kim, Hyeun Bum, Kim, Heebal, Choi, Sang Ho, Kim, Bong-Soo
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
Chromosomes
Chromosomes, Bacterial
Comparative analysis
Computational Biology - methods
Cytotoxicity
Fish
Fishes - microbiology
Food Microbiology
Gastroenteritis
Gene Order
Genome, Bacterial
Genomes
Genomics
Genomics - methods
Humans
Pathogens
Seafood
Sequence Analysis, DNA
Toxins
Vibrio Infections - microbiology
Vibrio parahaemolyticus - genetics
Vibrio parahaemolyticus - isolation & purification
Virulence Factors - genetics
Water
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Summary:Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that causes food-borne gastroenteritis in humans who consume V. parahaemolyticus-contaminated seafood. The FORC_023 strain was isolated from raw fish storage water, containing live fish at a sashimi restaurant. Here, we aimed to sequence and characterize the genome of the FORC_023 strain. The genome of the FORC_023 strain showed two circular chromosomes, which contained 4227 open reading frames (ORFs), 131 tRNA genes and 37 rRNA genes. Although the genome of FORC_023 did not include major virulence genes, such as genes encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), it contained genes encoding other hemolysins, secretion systems, iron uptake-related proteins and several V. parahaemolyticus islands. The highest average nucleotide identity value was obtained between the FORC_023 strain and UCM-V493 (CP007004-6). Comparative genomic analysis of FORC_023 with UCM-V493 revealed that FORC_023 carried an additional genomic region encoding virulence factors, such as repeats-in-toxin and type II secretion factors. Furthermore, in vitro cytotoxicity testing showed that FORC_023 exhibited a high level of cytotoxicity toward INT-407 human epithelial cells. These results suggested that the FORC_023 strain may be a food-borne pathogen. Determination of the genomic content of Vibrio parahaemolyticus FORC_023, isolated from raw fish storage water at sashimi restaurant, will provide additional information that will facilitate the study of pathogenesis in this important human pathogen. Graphical Abstract Figure. Determination of the genomic content of Vibrio parahaemolyticus FORC_023, isolated from raw fish storage water at sashimi restaurant, will provide additional information that will facilitate the study of pathogenesis in this important human pathogen.
ISSN:2049-632X
2049-632X
DOI:10.1093/femspd/ftw032