Assessment of urinary microparticles in normotensive patients with type 1 diabetes

Aims/hypothesis Assessment of urinary extracellular vesicles including exosomes and microparticles (MPs) is an emerging approach for non-invasive detection of renal injury. We have previously reported that podocyte-derived MPs are increased in diabetic mice in advance of albuminuria. Here, we hypoth...

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Bibliographic Details
Published in:Diabetologia 2017-03, Vol.60 (3), p.581-584
Main Authors: Lytvyn, Yuliya, Xiao, Fengxia, Kennedy, Christopher R. J., Perkins, Bruce A., Reich, Heather N., Scholey, James W., Cherney, David Z., Burger, Dylan
Format: Article
Language:English
Subjects:
Adult
Albuminuria - urine
Biomarkers
Biomarkers - urine
Blood Pressure - physiology
Creatinine
Creatinine - metabolism
Diabetes
Diabetes Mellitus, Type 1 - physiopathology
Diabetes Mellitus, Type 1 - urine
Extracellular vesicles
Flow Cytometry
Health care networks
Human Physiology
Humans
Hyperglycemia
Hyperglycemia - physiopathology
Hyperglycemia - urine
Internal Medicine
Male
Medicine
Medicine & Public Health
Membrane Proteins
Metabolic Diseases
Microscopy, Electron
Microscopy, Electron, Transmission
Morphology
Nanoparticles
Podocytes - metabolism
Podocytes - ultrastructure
Short Communication
Transmission electron microscopy
Urine
Young Adult
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Summary:Aims/hypothesis Assessment of urinary extracellular vesicles including exosomes and microparticles (MPs) is an emerging approach for non-invasive detection of renal injury. We have previously reported that podocyte-derived MPs are increased in diabetic mice in advance of albuminuria. Here, we hypothesised that type 1 diabetes and acute hyperglycaemia would increase urinary podocyte MP levels in uncomplicated diabetes. Methods In this post hoc exploratory analysis, we examined archived urine samples from normoalbuminuric patients with uncomplicated type 1 diabetes studied under clamped euglycaemia and hyperglycaemia and compared with healthy controls. Urinary vesicles were assessed by electron microscopy and nanoparticle tracking while podocyte MPs were assessed by flow cytometry. Results Neither vesicle size nor total number were significantly altered in type 1 diabetes or acute hyperglycaemia. By contrast, urinary podocyte MP levels were higher in type 1 diabetes (0.47 [0.00–3.42] MPs/μmol creatinine [Cr]) compared with healthy controls (0.00 [0.00–0.00] MPs/μmol Cr, p  
ISSN:0012-186X
1432-0428
DOI:10.1007/s00125-016-4190-2