Fast activated charcoal prepurification of Fusarium solani [beta]-glucosidase for an efficient oleuropein bioconversion

• Published in: Preparative biochemistry & biotechnology 2017-02, Vol.47 (2), p.185
• Main Authors: Boudabbous, Manel, Saibi, Walid, Bouallagui, Zouhaier, Dardouri, Mosbeh, Sayadi, Sami, Belghith, Hafedh, Mechichi, Tahar, Gargouri, Ali
• Format: Article
• Language: English
• Subjects: Biochemistry; Biotechnology; Charcoal
• ISSN: 1082-6068; 1532-2297
• DOI: 10.1080/10826068.2016.1201679

Fungal [beta]-glucosidases were extensively studied regarding their various potential biotechnology applications. Here, we report the selection of Fusarium solani strain producing high yield of [beta]-glucosidase activity. The effect of some factors on [beta]-glucosidase production was studied including: Initial pH, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. The optimal enzyme production was obtained with 4 units of pH. The highest [beta]-glucosidase activity was produced on 4% wheat bran (WB) as raw carbon sources, reaching 5 U/mL. A positive correlation between WB particle size and the [beta]-glucosidase production level was settled. The last one was enhanced to 13.60 U/mL in the presence of 0.5% (w/v) of ammonium sulfate. Interestingly, the activated charcoal was used as an inexpensive reagent enabling a rapid and efficient purification prior step that improved the enzyme-specific activity. Eventually, F. solani [beta]-glucosidase acts efficiently during the bioconversion process of oleuropein. Indeed, 82.5% of oleuropein was deglycosylated after 1 hr at 40°C. Altogether, our data showed that the [beta]-glucosidase of F. solani has a potential application to convert oleuropein to ameliorate food quality.