Chlorogenic Acid Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/NF-[kappa]B Signal Pathway

• Published in: Inflammation 2017-04, Vol.40 (2), p.523
• Main Authors: Ye, Han-yang, Jin, Jian, Jin, Ling-wei, Chen, Yan, Zhou, Zhi-hong, Li, Zhan-yuan
• Format: Article
• Language: English
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-016-0498-9

Chlorogenic acid (CGA), a polyphenolic compound, exists widely in medicinal herbs, which has been shown a strong antioxidant and anti-inflammatory effect. This study investigated the protective effects and mechanism of CGA on lipopolysaccharide (LPS)-induced acute kidney injury (AKI). Treatment of CGA successfully ameliorates LPS-induced renal function and pathological damage. Moreover, CGA dose-dependently suppressed LPS-induced blood urea nitrogen (BUN), creatinine levels, and inflammatory cytokines TNF-[alpha], IL-6, and IL-1[beta] in serum and tissue. The relative proteins' expression of TLR4/NF-[kappa]B signal pathway was assessed by western blot analysis. Our results showed that CGA dose-dependently attenuated LPS-induced kidney histopathologic changes, serum BUN, and creatinine levels. CGA also suppressed LPS-induced TNF-[alpha], IL-6, and IL-1[beta] production both in serum and kidney tissues. Furthermore, our results showed that CGA significantly inhibited the LPS-induced expression of phosphorylated NF-[kappa]B p65 and I[kappa]B as well as the expression of TLR4 signal. In conclusion, our results provide a mechanistic explanation for the anti-inflammatory effects of CGA in LPS-induced AKI mice through inhibiting TLR4/NF-[kappa]B signaling pathway.