A barley mutant with improved salt tolerance through ion homeostasis and ROS scavenging under salt stress

• Published in: Acta physiologiae plantarum 2017-03, Vol.39 (3), p.1-14, Article 90
• Main Authors: Kiani, Davood, Soltanloo, Hassan, Ramezanpour, Seyyede Sanaz, Nasrolahnezhad Qumi, Ali Asghar, Yamchi, Ahad, Zaynali Nezhad, Khalil, Tavakol, Elahe
• Format: Article
• Language: English
• Subjects: Abiotic stress; Agriculture; Ammonia; Barley; Biomedical and Life Sciences; Catalase; Cell walls; Chlorophyll; Controlled conditions; Gene expression; Genes; Genotypes; Homeostasis; Hydrogen peroxide; Life Sciences; Malondialdehyde; Original Article; Peroxidase; Phenylalanine; Phenylalanine ammonia-lyase; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Proline; Roots; Salinity tolerance; Salt; Salt tolerance; Salts; Scavenging; Shoots; Sodium; Sodium chloride; Stress; Stresses
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-017-2359-z

To investigate key regulatory components and genes with great impact on salt tolerance, near isogenic or mutant lines with distinct salinity tolerance are suitable genetic materials to simplify and dissect the complex genes networks. In this study, we evaluated responses of a barley mutant genotype (73-M4-30), in comparison with its wild-type background (Zarjou) under salt stress. Although the root growth of both genotypes was significantly decreased by exposure to sodium chloride (NaCl), the effect was greater in the wild type. The chlorophyll content decreased under salt stress for the wild type, but no change occurred in the mutant. The mutant maintained the steady-state level of [K + ] and significantly lower [Na + ] concentrations in roots and higher [K + ]/[Na + ] ratio in shoots under salt conditions. The catalase (CAT), peroxidase (POD) activity, and proline content were higher in the mutant than those in the wild type under controlled conditions. The soluble proline was higher after 24 h of salt stress in roots of the mutant but was higher after 96 h of salt stress in the wild type. The CAT and POD activity of the mutant increased under salt stress which was as a coincidence to lower levels of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) contents. The ratio of dry-to-fresh weight of the roots increased for the mutant under salt stress which was as a result of the higher phenylalanine ammonia-lyase (PAL) gene expression and peroxidase activity and involved in cell wall lignification. Consequently, it seems that ion homeostasis and increased peroxidase activity have led to salt tolerance in the mutant’s genotype.